Stem cell preparation for treating canine arthritis and preparation method thereof

A stem cell preparation and arthritic technology are applied in the field of stem cell preparation for treating canine arthritis and its preparation, which can solve the problems of limited treatment plan, no therapy can control pain, etc., and achieve significant curative effect, no toxic side effects, and high safety. Effect

Inactive Publication Date: 2017-11-24
FOSHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The complex reality limits the treatment options for OA, as there are currently no medical or non-surgical treatments that have proven efficacy in reversing or halting the progression of the disease, and no therapy can control pain due to long-term exacerbating factors

Method used

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  • Stem cell preparation for treating canine arthritis and preparation method thereof
  • Stem cell preparation for treating canine arthritis and preparation method thereof
  • Stem cell preparation for treating canine arthritis and preparation method thereof

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Embodiment Construction

[0021] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0022] 1. Preparation of canine UC-MSCs

[0023] Take Huatong jelly from the umbilical cord, digest it with collagenase type Ⅰ, filter it, centrifuge, resuspend the cells in the complete medium, transfer to a 35mm culture dish, and place it at 37°C, 5% CO 2 In the incubator, after 2 days of culture, PBS was washed and changed; the medium was changed every 3 days, and the cells were subcultured when the cells covered 80% to 90% of the culture dish, and the third-generation UC-MSCs with good growth conditions were taken to make stem cell injection. liquid;

[0024] 2. Construction of experimental animal OA model

[0025] 1. Grouping treatment: Select 8 healthy dogs ...

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Abstract

The invention discloses a stem cell preparation for treating canine arthritis and a preparation method thereof. The stem cell preparation comprises canine mesenchymal stem cells, canine serum albumin, hyaluronic acid and pharmaceutical salts. Injections of the mesenchymal stem cells are skillfully used, a knee joint cavity is positioned through B-mode ultrasound, the cells are injected into the knee joint cavity of an affected limb more accurately, and a traditional treatment method for the canine arthritis is broken. The stem cell preparation is high in stability, good in therapeutic effect, safe in using, suitable for being preserved and transported for a long time and free of toxic and side effects, and lays the foundation for being used clinically on a large scale.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a stem cell preparation for treating canine arthritis and a preparation method thereof. Background technique [0002] Nowadays, with the increase of companion animal dogs year by year, canine OA is also increasingly valued by humans. OA causes swelling, pain, deformation, and effusion in the dog's joints, which leads to a series of obstacles affecting motor function. According to the etiology of OA, it can be divided into primary and secondary OA. Elderly dogs often suffer from primary OA due to excessive exercise and bone aging. Secondary OA can be caused by trauma, wear and other reasons that affect the normal function of the joints. The complex reality limits the treatment options for OA. At present, there is no drug or non-surgical treatment that has a clear effect on reversing or stopping the progression of the disease, and there is no therapy that can control the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/42A61K47/36A61K9/08A61P19/02A61P29/00
Inventor 王丙云张贝莹詹小舒李东升罗冬章陈志胜计慧琴陈胜锋
Owner FOSHAN UNIVERSITY
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