Application of alpha-ketoglutaric acid to improvement of animal reproduction function deficit caused by high fat diet

A technology of ketoglutaric acid and high-fat diet, which is applied in application, animal feed, additional food elements, etc., can solve the problems affecting the popularization and application of α-ketoglutaric acid, estrus cycle disorder, ovarian shape and function, and sex hormone secretion and reproduction. Functional damage and other problems, to achieve the effect of improving reproductive function damage, not causing toxic and side effects, and improving reproductive function

Active Publication Date: 2017-12-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no research on the improvement of animal reproductive function damage caused by high-fat diets, especially α-ketoglutarate on the improvement of estrous cycle disorders, ovarian mor

Method used

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  • Application of alpha-ketoglutaric acid to improvement of animal reproduction function deficit caused by high fat diet
  • Application of alpha-ketoglutaric acid to improvement of animal reproduction function deficit caused by high fat diet
  • Application of alpha-ketoglutaric acid to improvement of animal reproduction function deficit caused by high fat diet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 α-ketoglutaric acid can alleviate the abnormal estrous cycle of mice fed with high fat

[0035] 1. In order to study the effect of α-ketoglutaric acid on the estrous cycle of high-fat fed mice, 10-week-old female C57BL / 6 mice were used as the research object.

[0036] 2. Experimental method

[0037] (1) Animal breeding method: 1 animal / box, single-breeding; feeding temperature and humidity: 22-26°C, 40%-70%; 12 h: 12 h day and night intermittent lighting; the conditions of the breeding room are always kept stable to ensure the test reliability of the results. During the breeding period, the animals had free access to food and drinking water, and the feed and drinking water were provided by Guangdong Medical Experimental Animal Center. The purchased SPF grade C57BL / 6 mice were pre-fed for 7 days. Animals were checked once a day during this period.

[0038](2) Animal grouping and treatment: 10-week-old mice were randomly divided into 3 groups: control group,...

Embodiment 2

[0043] Example 2 Effect of adding 2% α-ketoglutaric acid to drinking water on the morphology and function of mouse ovaries

[0044] 1. In order to study the effect of α-ketoglutaric acid on the nest shape and function of high-fat-fed mice, 10-week-old female C57BL / 6 mice were used as the research object.

[0045] 2. Experimental method

[0046] (1) Animal feeding method: same as in Example 1.

[0047] (2) Grouping and treatment of animals: the same as in Example 1.

[0048] (3) Sampling and detection: The experiment was carried out for 20 weeks. On the day of the end of the experiment, the mice were sacrificed by cervical dislocation, and the ovaries of 9 mice were collected for paraffin section and HE staining. To detect the effect of α-ketoglutarate on the number of corpus luteum and theca cell thickness in mouse ovary.

[0049] 3. The result is as follows figure 2 Shown:

[0050] (1) if figure 2 As shown in A, compared with the normal diet group (control group), the...

Embodiment 3

[0052] Example 3 Effect of Adding 2% α-Ketoglutaric Acid in Drinking Water on Serum Sex Hormones in Mice

[0053] 1. In order to study the effect of α-ketoglutarate on serum sex hormones in mice fed with high-fat diet, 10-week-old female C57BL / 6 mice were used as the research object.

[0054] 2. Experimental method

[0055] (1) Animal feeding method: same as in Example 1.

[0056] (2) Grouping and treatment of animals: the same as in Example 1.

[0057] (3) Sampling and detection: The experiment was carried out for 20 weeks. On the day of the end of the experiment, the mice were killed by cervical dislocation, blood was collected from the eyeballs, and the serum was separated. The effects of α-ketoglutarate on the serum sex hormones (follicle stimulating hormone, luteinizing hormone) levels in mice were detected by ELISA kit.

[0058] 3. The result is as follows image 3 Shown:

[0059] (1) if image 3 As shown in A, 3B and 3C, compared with the control group, the level ...

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Abstract

The invention discloses an application of alpha-ketoglutaric acid to improvement of animal reproduction function deficit caused by high fat diet, and provides a novel application of the alpha-ketoglutaric acid. Abnormity of animal estrus cycle, ovarian morphology and functions and sex hormone secretion caused by high fat diet can be remarkably improved by the alpha-ketoglutaric acid, animal reproduction function deficit caused by high fat diet is further improved, and particularly, estrus cycle disorder, corpus luteum number depletion, theca cell thickness increase, follicle-stimulating hormone content reduction and luteinizing hormone content rise caused by high fat diet are improved by the alpha-ketoglutaric acid, so that animal reproduction function deficit caused by high fat diet can be remarkably improved. Moreover, the alpha-ketoglutaric acid is free from substances harmful to animals or human bodies, adding quantity is small, and the alpha-ketoglutaric acid can be directly added into animal drinking water, feed, food or drugs, has the advantages of safety, effectiveness, no toxic and side effects and the like and has a good popularization and application prospect in improvement of animal reproduction function deficit caused by high fat diet.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction and development, and relates to the application of α-ketoglutarate in improving animal reproductive function damage caused by a high-fat diet, in particular to the application of α-ketoglutarate in improving estrus cycle disorder and ovarian Form and function and abnormal secretion of sex hormones and other aspects of the application. Background technique [0002] In daily life, production and clinical practice, animals and humans who eat high-fat food for a long time are often accompanied by a variety of metabolic diseases, such as insulin resistance, diabetes and polycystic ovary syndrome. Female animals that eat too much high-fat food often have symptoms such as abnormal estrus, high androgen, and ovulation disorders, which in turn affect their reproductive and reproductive functions. For example, studies have shown that sows with frequent high-fat diets will affect their estrus an...

Claims

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Application Information

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IPC IPC(8): A61K31/194A61P15/00A61P15/08A23K20/10
CPCA23K20/10A61K31/194
Inventor 王松波江青艳束刚张枫琳朱晓彤王丽娜高萍
Owner SOUTH CHINA AGRI UNIV
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