Application of α-ketoglutarate in improving animal reproductive function damage caused by high-fat diet
A high-fat diet and animal breeding technology, applied in the fields of application, animal feed, additional food elements, etc., can solve reproductive function damage, estrus cycle disorder, ovarian shape and function, and sex hormone secretion, and affect the popularization and application of α-ketoglutarate To achieve the effect of improving reproductive function damage, safe and easy to obtain, and not causing toxic and side effects
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Embodiment 1
[0032] Example 1 α-ketoglutarate can alleviate the abnormality of estrous cycle in mice fed with high fat
[0033] 1. In order to study the effect of α-ketoglutaric acid on the estrous cycle of high-fat fed mice, 10-week-old female C57BL / 6 mice were used as the research object.
[0034] 2. Experimental method
[0035] (1) Animal feeding method: 1 animal / box, single breeding; feeding temperature and humidity: 22-26°C, 40%-70%; adopt 12h:12h day and night intermittent lighting; the conditions of the breeding room are always kept stable to ensure the test results reliability. During the breeding period, the animals had free access to food and drinking water, and the feed and drinking water were provided by Guangdong Medical Experimental Animal Center. The purchased SPF grade C57BL / 6 mice were pre-fed for 7 days. Animals were checked once a day during this period.
[0036] (2) Animal grouping and treatment: 10-week-old mice were randomly divided into three groups: control grou...
Embodiment 2
[0041] Example 2 Effect of Adding 2% α-Ketoglutaric Acid in Drinking Water on Ovarian Morphology and Function of Mice
[0042] 1. In order to study the effect of α-ketoglutaric acid on the nest shape and function of high-fat-fed mice, 10-week-old female C57BL / 6 mice were used as the research object.
[0043] 2. Experimental method
[0044] (1) Animal breeding method: the same as in Example 1.
[0045] (2) Grouping and treatment of animals: same as in Example 1.
[0046] (3) Sampling and detection: The experiment was carried out for 20 weeks. On the day of the end of the experiment, the mice were killed by cervical dislocation, and the ovaries of 9 mice were collected for paraffin section and HE staining. To detect the effect of α-ketoglutarate on the number of corpus luteum and theca cell thickness in mouse ovary.
[0047] 3. The result is as follows figure 2 Shown:
[0048] (1) if figure 2 As shown in A, compared with the normal diet group (control group), the number ...
Embodiment 3
[0050] Example 3 Effect of Adding 2% α-Ketoglutaric Acid in Drinking Water on Serum Sex Hormones in Mice
[0051] 1. In order to study the effect of α-ketoglutarate on serum sex hormones in mice fed with high-fat diet, 10-week-old female C57BL / 6 mice were used as the research object.
[0052] 2. Experimental method
[0053] (1) Animal breeding method: the same as in Example 1.
[0054] (2) Grouping and treatment of animals: same as in Example 1.
[0055] (3) Sampling and detection: The experiment was carried out for 20 weeks. On the day of the end of the experiment, the mice were killed by cervical dislocation, blood was collected from the eyeballs, and the serum was separated. The effects of α-ketoglutarate on serum sex hormones (follicle-stimulating hormone, luteinizing hormone) levels in mice were detected by ELISA kit.
[0056] 3. The result is as follows image 3 Shown:
[0057] (1) if image 3 As shown in A, 3B and 3C, compared with the control group, the level of ...
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