Primers, probes and methods for screening leukemia mll-sept6 fusion gene by real-time fluorescent PCR

A technology of real-time fluorescence and gene fusion, which is applied in the field of biotechnology detection, can solve the problems that karyotype analysis cannot satisfy MRD, sensitivity cannot be satisfied at the same time, and there are many PCR reaction systems, so as to avoid hematological recurrence, save detection time, and prevent The effect of clinical recurrence
CN107447029BActive Publication Date: 2021-02-23北京艾迪康医学检验实验室有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京艾迪康医学检验实验室有限公司
Publication Date
2021-02-23

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Abstract

The invention discloses a method for detecting the relative expression of MLL-SEPT6 fusion gene by using fluorescent quantitative PCR technology, including specific primers and probes, which can be used as a screening method for the trace residue of MLL-SEPT6 fusion gene in leukemia patients, so as to prevent timely intervention and treatment. Hematological recurrence, adjustment of treatment plan, evaluation of treatment effect, prediction of prognosis and prevention of clinical recurrence are all of great significance.
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Description

technical field

[0001] The invention belongs to the field of biotechnology detection, and particularly relates to a method for screening fusion genes, which uses probe Taqman real-time fluorescent PCR technology to detect the situation of MLL-SEPT6 fusion genes in human leukemia patients. Background technique

[0002] Leukemia is a kind of clonal malignant disease with abnormal hematopoietic stem cells. The leukemia cells in its clones lose the ability to further differentiate and mature and stagnate at different stages of cell development. In the bone marrow and other hematopoietic tissues, a large number of leukemia cells proliferate and accumulate and infiltrate other organs and tissues, and at the same time inhibit normal hematopoiesis. The clinical manifestations are anemia, bleeding, infection and infiltration of various organs. The etiology and pathogenesis of human leukemia are still not fully understood. The known causes include infectious factors, ionizing radiat...

Claims

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