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Skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof

A technology of skin repair agent and extract, which is applied in skin diseases, drug combinations, medical preparations containing active ingredients, etc., to reduce the occurrence of scars, increase blood vessel density, and promote skin repair effects

Active Publication Date: 2018-01-09
上海亚睿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is currently no report on mesenchymal stem cell extracts combined with traditional Chinese medicine extracts to promote skin repair and reduce scar formation

Method used

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  • Skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof
  • Skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof
  • Skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The preparation of embodiment 1 skin restoration agent of the present invention (1)

[0072] Step 1: Preparation of umbilical cord mesenchymal stem cell extract

[0073] 1) Take out the umbilical cord tissue of the newborn, remove blood vessels, epithelial membranes and blood clots, separate Wharton's blocks, and cut them into 1mm 3 After size, put it into a 10cm sterile petri dish; digest with DMEM medium containing 0.1% collagenase II; after filtration, use DMEM medium containing 10% fetal bovine serum FBS for subculture, and replace it with serum-free medium on the third day Subculture in DMEM medium, once every 2 days; take a bottle of cells from passage 3 for subsequent treatment;

[0074] 2) digest the cells with DMEM medium containing 0.05M EDTA, and wash with PBS solution;

[0075] 3) The cells were resuspended in serum-free DMEM medium, and lysed and crushed by ultrasonic (120W, 15S*20 times, 15S interval), the crushed product was centrifuged at 12000rpm for ...

Embodiment 2

[0088] Cytotoxicity test of embodiment 2 skin restoration agent of the present invention

[0089] 1. Experimental method

[0090] 1) Primary culture of umbilical cord mesenchymal stem cells;

[0091] 2) The umbilical cord mesenchymal stem cells were mixed with 2*10 5 cells / ml into a 12-well plate for experiments, and adherent culture for at least 24 hours;

[0092] 3) adding the skin repair agent according to the present invention (mass concentration is 0%, 0.5%, 1%, 2%, 4%, 10% and 15%), and adding the cell damage agent bleomycin as a control;

[0093] 4) Perform flow cytometric detection on the cells to detect cell apoptosis and cell viability (CCK8 method).

[0094] 2. Experimental results

[0095] For the CCK8 experiment of mesenchymal stem cells, the cytotoxicity of the skin repair agent of the present invention at 4% concentration is 4.2%, and the cytotoxicity at 10% concentration is 14.5%, showing that its cytotoxicity is small, and 4% mass concentrations for subse...

Embodiment 3

[0096] Example 3 The cell experiment that the skin repair agent of the present invention inhibits the growth of fibroblasts and promotes the growth of epidermal stem cells and vascular endothelial cells

[0097] 1. Experimental method

[0098] 1) Primary culture of different cells, including fibroblasts, epidermal stem cells and vascular endothelial cells;

[0099] 2) Divide cells into 2*10 5 Spread into 12-well plates for experiments, 4 wells for each type of cells, and culture on the wall for at least 24 hours;

[0100] 3) Add the skin repairing agent (mass concentration 4%) of the present invention to each kind of cell respectively, and add bleomycin (final concentration is 2 μ g / ml) as the cell damage agent, the specific adding method is:

[0101]

[0102] 4) Perform flow cytometry detection on all cells to detect cell apoptosis and cell viability (CCK8 method).

[0103] 2. Experimental results

[0104]The results of cell apoptosis in each group are shown in Table 1...

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Abstract

The invention relates to a skin repairing agent prepared by mixing stem cell extract and traditional Chinese medicine extract. A preparation method of the skin repairing agent comprises the followingsteps: (1) culturing umbilical cord mesenchymal stem cells and preparing extract by utilizing technologies including ultrafiltration and the like; (2) extracting total flavonoids of traditional Chinese medicines including rhizoma phragmitis, blue lotus flowers, hibiscus flowers and liquorice roots; (3) mixing the stem cell extract and the traditional Chinese medicine extract. The skin repairing agent provided by the invention can act on parts including scars, stretch marks and the like through methods including spraying, injection and the like; the scars, the stretch marks and the like are treated through promoting tissue revitalization and killing fibroblasts; the skin repairing agent has the advantages of simple method, high efficiency and small side effects; a production technology of the preparation is simple and the cost is low.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a skin repair agent prepared by mixing stem cell extracts and traditional Chinese medicine extracts and its application. Background technique [0002] Scar is a natural phenomenon that occurs in the epidermis of animals. It is a pathological phenomenon that must occur during post-traumatic repair, which will lead to changes in the appearance and structure of the skin. Scars have certain negative effects in wound repair, which not only lead to the destruction of skin appearance, but also may cause functional impacts. It can be said that scars have both psychological and physical negative effects on the recovery of the human body after trauma. However, there is still a lack of very effective treatments for scars. Among the common methods, pressure therapy has limited effect and requires long-term persistence, chemotherapy and radiation therapy have obvious side effects, and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K36/899A61P17/02
Inventor 童强汪佳祺
Owner 上海亚睿生物科技有限公司
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