Method for naked eye discernible colorimetric detection on dimethoate

A technology of dimethoate and naked eye, which is applied to the measurement of color/spectral characteristics, material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problems of long detection time, poor specificity, and processing Complicated problems, to achieve the effect of low detection cost, visible to the naked eye, and good selectivity

Inactive Publication Date: 2018-01-09
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the existing detection methods have the disadvantages of complex processing, low sensitivity, poor specificity, high cost, and long detection time.

Method used

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  • Method for naked eye discernible colorimetric detection on dimethoate
  • Method for naked eye discernible colorimetric detection on dimethoate
  • Method for naked eye discernible colorimetric detection on dimethoate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 of the present invention: a kind of method that the naked eye can distinguish colorimetric detection dimethoate comprises the following steps:

[0042] A: Draw a standard curve of dimethoate absorbance change

[0043] A1-1: Prepare the detection solution of known dimethoate concentration: take 16 1.5mL graduated centrifuge tubes, add 5.0μL of ssDNA mother solution with a concentration of 3μM and 50.0μL of AuNPs solution with a concentration of 56.4nM, and mix well Incubate at 30°C for 30 minutes, then add 10 μL of a dimethoate standard solution of known concentration to each graduated centrifuge tube, mix well and incubate at 30°C for 30 minutes to obtain product A1-1;

[0044]A1-2: Add 415 μL of 8 mM sodium acetate buffer solution to A1-1, and finally add 10.0 μL of 5 M H 2 o 2 The solution and 10.0 μL of OPD solution with a concentration of 800 mM were thoroughly mixed to obtain 500 μL of detection solution, which was product A1.

[0045] A2: Preparati...

Embodiment 2

[0061] Embodiment 2 of the present invention: a kind of method that the naked eye can distinguish colorimetric detection dimethoate comprises the following steps:

[0062] A: Draw a standard curve of dimethoate absorbance change

[0063] A1-1: Prepare the detection solution with known dimethoate concentration: take 16 1.5mL graduated centrifuge tubes, add 4.0μL of 3μM ssDNA mother solution and 47.5μL of 56.4nM AuNPs solution, mix well Incubate at 25°C for 35 minutes, then add 10 μL of a dimethoate standard solution of known concentration to each graduated centrifuge tube, mix well and incubate at 25°C for 35 minutes to obtain product A1-1;

[0064] A1-2: Add 405 μL of 8 mM sodium acetate buffer solution to A1-1, and finally add 8.5 μL of 5 M H 2 o 2 The solution and 8.5 μL of OPD solution with a concentration of 800 mM were thoroughly mixed to obtain a detection solution, which was product A1.

[0065] A2: Preparation of blank control solution: Take another graduated centri...

Embodiment 3

[0074] Embodiment 3 of the present invention: a kind of method that the naked eye can distinguish colorimetric detection dimethoate comprises the following steps:

[0075] A: Draw a standard curve of dimethoate absorbance change

[0076] A1-1: Prepare the detection solution with known dimethoate concentration: take 16 1.5mL graduated centrifuge tubes, add 6.0μL of 3μM ssDNA mother solution and 52.5μL of 56.4nM AuNPs solution, and mix well Incubate at 35°C for 25 minutes, then add 10 μL of a dimethoate standard solution of known concentration to each graduated centrifuge tube, mix well, and incubate at 35°C for 25 minutes to obtain product A1-1;

[0077] A1-2: Add 425 μL of 8 mM sodium acetate buffer solution to A1-1, and finally add 11.5 μL of 5 M H 2 o 2 The solution and 11.5 μL of OPD solution with a concentration of 800 mM were thoroughly mixed to obtain a detection solution, which was product A1.

[0078]A2: Preparation of blank control solution: Take another graduated ...

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Abstract

The invention discloses a method for naked eye discernible colorimetric detection on dimethoate. The method comprises the following steps: A, drawing a dimethoate absorbancy variation standard curve;B, according to the dimethoate absorbancy variation standard curve, establishing a linear regression equation according to the mutual relationship between dimethoate concentration and dimethoate absorbancy values; C, preparing a solution C to be detected with unknown dimethoate concentration; D, performing absorption spectrum scanning on the solution C, testing the absorbancy value of the solutionC at the wavelength of 450nm, calculating an absorbancy value difference of the solution C and a product A, and substituting the absorbancy value difference into the linear regression equation, thereby obtaining the concentration of dimethoate in the solution C. The method needs no large-size instrument equipment in the application process, has the advantages of high detection sensitivity, good selectivity, simple and rapid operation, naked eye discrimination and the like and can be widely applied to rapid detection on dimethoate residues in agricultural products.

Description

technical field [0001] The invention relates to a method for detecting dimethoate, in particular to a method for detecting dimethoate with visible colorimetry. Background technique [0002] Dimethoate is an important organophosphorus pesticide, which has a strong inhibitory effect on cholinesterase in the organism, making cholinesterase lose the ability to decompose acetylcholine, resulting in a large accumulation of acetylcholine, thereby blocking nerve conduction , causing central nervous system poisoning. At present, dimethoate is widely used in vegetables, fruit trees, tea, mulberry, cotton, oil plants, food and other crops. However, the excessive use of dimethoate has resulted in a large amount of pesticide residues in fruits and oil crops, which has caused serious harm to human health. Therefore, it is very important to establish a method for rapid detection of dimethoate. [0003] At present, the methods for detecting dimethoate mainly include instrumental analysis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 吴远根陈华云胡阳杨文平赵静
Owner GUIZHOU UNIV
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