41results about How to "Detection without interference" patented technology

The invention discloses polystyrene mercury ion fluorescence recognition materials. The structural formula of the polystyrene mercury ion fluorescence recognition materials is as follows. The invention also discloses a preparation method of the functionalized polystyrene mercury ion fluorescence recognition materials. The preparation method comprises the following specific implementation steps of, preparing 5-amino-2-(4'-hydroxy-3'-(2-(2''-ethylsuleenyl ethyl) amino) phenyl-5-amino benzo [d] oxazole (VII), preparing aldehyde polystyrene microspheres (IX) and performing reaction preparation on the compounds (VII) and (IX) obtained through preparation to obtain the functionalized polystyrene mercury ion fluorescence recognition materials (X). The detection sensitivity of the polystyrene mercury ion fluorescence recognition materials is greatly improved due to the molecular wire effect of a polymer and the detection limit is 0.006 micromole every liter. The polystyrene mercury ion fluorescence recognition materials can enter cells and can be applied to the detection on mercury ions in the cells.

The invention provides a kit for co-separation of (deoxyribonucleic acid) and RNA (ribonucleic acid) from FFPE (formalin fixed and paraffin embedded tissues) as well as a method. The kit can be used for simultaneously extracting DNA and RNA from an FFPE sample, and has the advantages that not only can nucleic acid extraction be performed simultaneously, time is saved, and the extraction is economical and fast, but also the sample size can be saved, the problem of sample lack in a test can be solved effectively, and further, identical source of the DNA and the RNA used in study can be guaranteed. The kit and the method have the advantages that the operation is simple and direct, the repeatability is high, the yield is high, and enough DNAs and RNAs can be obtained simultaneously only through 2-5 pieces of tumor tissue sections; the obtained nucleic acids are high in purity and has no interference to follow-up detection; and compared with the common kit, the kit has remarkable advantages.

The invention discloses an electrochemical biosensor based on polypeptide analog with the electrocatalytic activity for acetylcholin esterase detection. The biosensor for acetylcholin esterase detection is characterized by comprising the following steps: (1) intensively stirring and uniformly mixing silver nitrate, L-cysteine and secondary distilled water on a stirrer, and incubating in an oscillator; (2) electrodepositing graphene onto a bare glassy carbon electrode by utilizing an electrochemical method, mixing 0.05 weight percent of a Nafion solution with the solution obtained in the step (1), and dropwise coating GO/GCE to obtain an electrochemical biosensor based on the polypeptide analog with electrocatalytic activity (CP/GO/GCE); (3) performing acetylcholin esterase activity detection, namely catalyzing acetylcholine in one step to generate H2O2, uniformly mixing acetylcholine, AchE and choline oxidase, and incubating to generate H2O2, and detecting the solution with the sensorprepared in the step (2) with good specificity, high sensitivity, high detection speed, accurate and reliable result; and (4) performing AChE inhibitor malathion detection, namely uniformly mixing acetylcholine, AChE, malathion and choline oxidase, incubating, and detecting the solution with the sensor prepared in the step (2). The biosensor has the advantages of good specificity, high sensitivity, high detection speed and accurate and reliable result.

The invention discloses a method for simultaneously determining 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and related substances thereof. According to the method, the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and the related substances thereof are separated through high performance liquid chromatography, wherein a chromatographic column is bonded by octadecyl silica gel; a mobile phase is a methanol:acetic acid aqueous solution. The flow velocity in the high performance liquid chromatography is 0.5 to 1.2ml/min. The detection wavelength in the high performance liquid chromatography is 226nm. The method is simple, convenient and practical, can be used for quickly achieving the aims of simultaneously determining and separating the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione and the related substances thereof, can be applied to determination of molecular weights of unknown impurities through liquid chromatography-mass spectrometry (LC-MS), provides a reliable basis for structure inference of the unknown impurities, and facilitates quality control of raw materials and preparations of the 4-amino-2-(2,6-dioxo-3-piperidyl)isoindoline-1,3-dione.

The invention relates to a rare earth-organic porous material, a preparation method thereof and application thereof in the detection of nerve disease marker glutamic acid. Through the technical scheme, the preparation method comprises the following steps: dissolving Ln(NO3)3.6H2O, hydroxyterephthalic acid and 2-fluorobenzoic acid into a mixed solution of an organic solvent, water and nitric acid;sealing after ultrasonic uniformity, putting the mixture in a baking oven at the temperature of 110 DEG C for 3 d; naturally cooling to room temperature, washing, filtering and drying to obtain the rare earth-organic porous material. The rare earth-organic porous material prepared in the invention has obvious fluorescent response to the nerve disease marker glutamic acid, rare earth luminescence is taken as an interior label, the luminescence of organic molecules is taken as a detection signal, high-selective detection for glutamic acid can be realized, and then the material can be used for early prevention and diagnosis of nerve diseases.

The invention relates to the technical field of single-chip microcomputers, in particular to a micro-fluidic chip for detecting micro-pollutants, which is characterized by comprising a first acrylic plate and a second acrylic plate, nine engraved grooves are formed in the first acrylic plate and used for storing detection liquid and providing detection space, the first groove to the seventh grooveare used for storing detected objects and objects to be detected, the eighth groove is used for storing waste liquid, and the ninth groove is used for reacting. The device is small, exquisite, portable, simple in structure, low in cost, high in stability, long in service lifetime and free of interference to detection.

The invention discloses a fluorescent colorimetric chemical-sensitive material as well as a synthesis method and application of the material. The material has the following structural formula shown in the specification, wherein R<1>, R<2>, R><3>, R<4>, R<5>, R<6>, R<7>, R<8> and R<9> are selected from hydrogen atom, C1-18 alkyl, aryl, ester group or ether group; Ar is selected from thiophene, pyrrole, benzene, naphthalene, anthracene, pyrene, indole, coumarin, fluorescein, carbazole, rhodamine, cyano dye, fluorene or quinoline; m is 1-1000; n is 1-1000; and X<-> is selected F<->, Cl<->, Br<->, or I<->. The compound can be used for detecting existence and content of lipopolysaccharide.

The invention belongs to the field of detection, and provides a method for rapidly identifying and detecting a main allergen, tropomyosin, in an aquatic product based on an intermediate infrared spectrum technology. By obtaining an intermediate infrared spectrogram of a detected sample, through second-order derivation, the infrared spectrogram characteristic peak of the tropomyosin and the infrared spectrogram of the detected sample are compared, and thus rapid qualitative identification and quantitative detection of the tropomyosin are achieved. The tropomyosin can be rapidly detected withoutantibodies, the purposes of rapid qualitative identification and quantitative detection can further be achieved, and meanwhile the requirement of non-destructive food is met.

The invention discloses a test paper for divalent manganese ions as well as a preparation method and application thereof. The preparation method of the test paper comprises steps that: a) sodium carboxymethylcellulose (CMC-Na) is used to react with TbCl3 to prepare CMC/Tb3 + complex; b) purified CMC/Tb3 + complex is prepared into solution, and is coated on non-fluorescent paper, and then dried. The tested solution is dripped on the test paper, and the test paper is observed under ultraviolet light, and then whether the test paper contains divalent manganese ions and the corresponding concentration range thereof can be determined; and at last, the detection limit is that the manganese ion is more than 0.0050 mg/L. The test paper for the divalent manganese ions is simple and convenient to apply and operate, high in sensitivity and capable of achieving rapid detection.

The invention discloses a paint concentration detecting device which comprises a machine body, wherein an air inlet pipe is fixed to the upper surface wall of the machine body; a warning light, a liquid crystal display, a control button and an alarm are arranged on the front surface wall of the machine body from top to bottom in sequence; an induced draft fan is arranged inside the machine body; afan impeller arranged inside the induced draft fan is fixedly connected with a main shaft of an air guiding motor; the air guiding motor and the machine body are fixedly connected by a motor fixing frame; an induced draft pipe fixed on the right surface wall of the induced draft fan and a color developing agent solution bottle are tightly connected by using a rubber plug; an infrared laser component arranged on the left side of the color developing agent solution bottle and the machine body are fixedly connected by an infrared laser component fixing bracket. The infrared laser component is arranged inside the paint concentration detecting device for irradiating a color developing agent solution with an infrared laser, so that the detection time is short, the reaction is carried out in onestep and the result is accurate, and other gases do not interfere with the detection.