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Molecularly imprinted membrane detecting device and preparation and detection methods thereof for tetracycline

A technology of molecularly imprinted membranes and detection devices, which is applied to measuring devices, analyzing materials, and analyzing materials through electromagnetic means. It can solve the problems of time-consuming, labor-intensive operations, complex operations, and low sensitivity, and achieve low testing costs and simple operations. Effect

Active Publication Date: 2010-02-17
广东盈峰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have deficiencies in actual detection, especially due to the complexity of environmental samples, traditional detection methods have problems such as complex operation, low sensitivity or time-consuming and laborious, which make them difficult to meet the requirements of on-site detection or portable detection

Method used

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  • Molecularly imprinted membrane detecting device and preparation and detection methods thereof for tetracycline
  • Molecularly imprinted membrane detecting device and preparation and detection methods thereof for tetracycline

Examples

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Effect test

Embodiment 1

[0024] A method for preparing a molecularly imprinted membrane detection device for tetracycline according to the present invention. The specific implementation method is as follows: the electrode substrate is electrodeposited titanium sheet with platinum particles with micro-nano structure, the titanium sheet is polished to smooth, and then placed in ethanol respectively Ultrasonic cleaning with deionized water; the direct current electrodeposition method is used to deposit platinum particles, and the electrolyte is composed of chloroplatinic acid solution containing hydrochloric acid; 400, 600, 800, and 1,000-mesh sandpaper were polished until smooth, and then placed in ethanol and deionized water for 3 and 20 minutes of ultrasonication respectively; DC electrodeposition was used to deposit platinum particles, and the electrolyte composition was 2g / L hydrochloric acid containing 1.2mmol / L Chloroplatinic acid solution in L. Deposition voltage was -0.35V, deposition was 30 minu...

Embodiment 2

[0029] A method for preparing a molecularly imprinted membrane detection device for tetracycline of the present invention, its specific implementation method is (the preparation method of the electrode base is as in Example 1):

[0030] Tetracycline molecularly imprinted membrane electrode processing process: template molecules, functional monomers, cross-linking agents, initiators, porogens are mixed and ultrasonically dissolved, then polymerized on the electrode substrate by photopolymerization, and the electrode substrate is impregnated into the sol, slowly Pull it out, seal it into a quartz tube, and fill it with nitrogen to remove the oxygen in it; finally, place it under a UV lamp, light and polymerize on both sides at the same time, and prepare an electrode covered with a molecularly imprinted polymer film; The electrode matrix is ​​respectively soaked in ethanol and water for 2-6 hours to elute template molecules therein, thereby obtaining a tetracycline molecularly imp...

Embodiment 3

[0034] A method for preparing a molecularly imprinted membrane detection device for tetracycline according to the present invention, its specific implementation mode 3 is (the preparation method of the electrode base is as in Example 1):

[0035] Tetracycline molecularly imprinted membrane electrode processing process: template molecules, functional monomers, cross-linking agents, initiators, porogens are mixed and ultrasonically dissolved, then polymerized on the electrode substrate by photopolymerization, and the electrode substrate is impregnated into the sol, slowly Pull it out, seal it into a quartz tube, and fill it with nitrogen to remove the oxygen in it; finally, place it under a UV lamp, light and polymerize on both sides at the same time, and prepare an electrode covered with a molecularly imprinted polymer film; The electrode matrix is ​​respectively soaked in ethanol and water for 2-6 hours to elute template molecules therein, thereby obtaining a tetracycline molec...

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Abstract

The invention discloses a method for preparing a molecularly imprinted membrane detecting device for tetracycline. The detecting device comprises a reactor, wherein the reactor is provided with a working electrode and a reference electrode; the working electrode is the tetracycline molecularly imprinted membrane electrode which comprises an electrode matrix; the reference electrode is a saturated calomel electrode; the electrode matrix is made by electrically depositing titanium sheets with micro-nano structure platinum particles; and the machining process of the tetracycline molecularly imprinted electrode includes: mixing materials such as template molecules and polymerzing on the electrode matrix through a photopolymerization method after ultrasound dissolve; dipping the electrode matrix in a sol, then eluting the template molecules therein, thereby preparing the electrode. The invention utilizes the identity of molecularly imprinted polymers (MIPs) to identify tetracycline special contamination without large-sized test apparatuses, has low test cost and simple and portable operation and has obvious advantages in on-site detection or portable detection aspects. The test technology can be widely applied to detecting tetracycline antibiotic special contamination contained in food, waste water of pharmaceutical factories, chemical plants, dye factories and the like.

Description

technical field [0001] The invention relates to a detection device and a preparation method using a molecularly imprinted membrane, in particular to a molecularly imprinted membrane detection device for tetracycline and a method thereof. Background technique [0002] Antibiotic residues accumulate in animals, and human consumption can cause allergic reactions and poisoning, or cause the body to develop drug resistance. Excessive use of antibiotics will lead to drug resistance of sensitive bacteria and reduce the efficacy of drugs. Drug residues also have teratogenic, mutagenic, carcinogenic and hormonal effects. Antibiotics and their metabolites enter the environment through feces and urine, are enriched by animals and plants, enter the food chain, and endanger human health. Tetracycline antibiotics are widely used as feed drug additives to prevent intestinal infection and promote growth of aquatic products. There are as many as 23 kinds of antibiotics added in feed, of wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/413G01N27/30C08F222/14C08F212/08C08F2/44C08J9/26
Inventor 王富生何姝王小仙廖小卿
Owner 广东盈峰科技有限公司
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