Molecularly imprinted membrane detecting device and preparation and detection methods thereof for tetracycline

Abstract

The invention discloses a method for preparing a molecularly imprinted membrane detecting device for tetracycline. The detecting device comprises a reactor, wherein the reactor is provided with a working electrode and a reference electrode; the working electrode is the tetracycline molecularly imprinted membrane electrode which comprises an electrode matrix; the reference electrode is a saturated calomel electrode; the electrode matrix is made by electrically depositing titanium sheets with micro-nano structure platinum particles; and the machining process of the tetracycline molecularly imprinted electrode includes: mixing materials such as template molecules and polymerzing on the electrode matrix through a photopolymerization method after ultrasound dissolve; dipping the electrode matrix in a sol, then eluting the template molecules therein, thereby preparing the electrode. The invention utilizes the identity of molecularly imprinted polymers (MIPs) to identify tetracycline special contamination without large-sized test apparatuses, has low test cost and simple and portable operation and has obvious advantages in on-site detection or portable detection aspects. The test technology can be widely applied to detecting tetracycline antibiotic special contamination contained in food, waste water of pharmaceutical factories, chemical plants, dye factories and the like.

Description

technical field

[0001] The invention relates to a detection device and a preparation method using a molecularly imprinted membrane, in particular to a molecularly imprinted membrane detection device for tetracycline and a method thereof. Background technique

[0002] Antibiotic residues accumulate in animals, and human consumption can cause allergic reactions and poisoning, or cause the body to develop drug resistance. Excessive use of antibiotics will lead to drug resistance of sensitive bacteria and reduce the efficacy of drugs. Drug residues also have teratogenic, mutagenic, carcinogenic and hormonal effects. Antibiotics and their metabolites enter the environment through feces and urine, are enriched by animals and plants, enter the food chain, and endanger human health. Tetracycline antibiotics are widely used as feed drug additives to prevent intestinal infection and promote growth of aquatic products. There are as many as 23 kinds of antibiotics added in feed, of wh...

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