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Preparation method of molecularly imprinted membrane detecting device for tetracycline

A molecularly imprinted membrane and detection device technology, which is applied to measurement devices, analytical materials, material analysis by electromagnetic means, etc., can solve the problems of complex operation, time-consuming and laborious, difficult to meet on-site detection or portable detection, etc., and achieve simple operation. , The effect of low test cost

Active Publication Date: 2013-03-27
广东盈峰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have deficiencies in actual detection, especially due to the complexity of environmental samples, traditional detection methods have problems such as complex operation, low sensitivity or time-consuming and laborious, which make them difficult to meet the requirements of on-site detection or portable detection

Method used

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  • Preparation method of molecularly imprinted membrane detecting device for tetracycline
  • Preparation method of molecularly imprinted membrane detecting device for tetracycline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a molecularly imprinted membrane detection device for tetracycline according to the present invention. The specific implementation method is as follows: the electrode substrate is electrodeposited titanium sheet with platinum particles with micro-nano structure, the titanium sheet is polished to smooth, and then placed in ethanol respectively Ultrasonic cleaning with deionized water; the direct current electrodeposition method is used to deposit platinum particles, and the electrolyte is composed of chloroplatinic acid solution containing hydrochloric acid; 400, 600, 800, and 1,000-mesh sandpaper were polished until smooth, and then placed in ethanol and deionized water for 3 and 20 minutes of ultrasonication respectively; DC electrodeposition was used to deposit platinum particles, and the electrolyte composition was 2g / L hydrochloric acid containing 1.2mmol / L Chloroplatinic acid solution in L. Deposition voltage was -0.35V, deposition was 30 minu...

Embodiment 2

[0027]A method for preparing a molecularly imprinted membrane detection device for tetracycline of the present invention, its specific implementation method is (the preparation method of the electrode base is as in Example 1):

[0028] Tetracycline molecularly imprinted membrane electrode processing process: template molecules, functional monomers, cross-linking agents, initiators, porogens are mixed and ultrasonically dissolved, then polymerized on the electrode substrate by photopolymerization, and the electrode substrate is impregnated into the sol, slowly Pull it out, seal it into a quartz tube, and fill it with nitrogen to remove the oxygen in it; finally, place it under a UV lamp, light and polymerize on both sides at the same time, and prepare an electrode covered with a molecularly imprinted polymer film; The electrode matrix is ​​respectively soaked in ethanol and water for 2-6 hours to elute template molecules therein, thereby obtaining a tetracycline molecularly impr...

Embodiment 3

[0032] A method for preparing a molecularly imprinted membrane detection device for tetracycline according to the present invention, its specific implementation mode 3 is (the preparation method of the electrode base is as in Example 1):

[0033] Tetracycline molecularly imprinted membrane electrode processing process: template molecules, functional monomers, cross-linking agents, initiators, porogens are mixed and ultrasonically dissolved, then polymerized on the electrode substrate by photopolymerization, and the electrode substrate is impregnated into the sol, slowly Pull it out, seal it into a quartz tube, and fill it with nitrogen to remove the oxygen in it; finally, place it under a UV lamp, light and polymerize on both sides at the same time, and prepare an electrode covered with a molecularly imprinted polymer film; The electrode matrix is ​​respectively soaked in ethanol and water for 2-6 hours to elute template molecules therein, thereby obtaining a tetracycline molec...

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Abstract

The invention discloses a molecularly imprinted membrane detecting device for tetracycline, which comprises a reactor, wherein the reactor is provided with a working electrode and a reference electrode; the working electrode comprises an electrode matrix which is formed by electrodepositing a platinum grain titanium sheet of a micro-nano structure; the reference electrode is a saturated calomel electrode; and the working electrode is a tetracycline molecularly imprinted membrane electrode. The invention also discloses the preparation and detection method of the molecularly imprinted membrane detecting device. The invention utilizes the identity of molecularly imprinted polymers (MIPs) to identify tetracycline special contamination without large-sized test apparatuses, has low test cost andsimple and portable operation and has obvious advantages in on-site detection or portable detection aspects. The test technology can be widely applied to detecting tetracycline antibiotic special contamination contained in food, waste water of pharmaceutical factories, chemical plants, dye factories and the like.

Description

technical field [0001] The invention relates to a method for preparing a detection device using a molecularly imprinted membrane, in particular to a method for preparing a molecularly imprinted membrane detection device for tetracycline. Background technique [0002] Antibiotic residues accumulate in animals, and human consumption can cause allergic reactions and poisoning, or cause the body to develop drug resistance. Excessive use of antibiotics will lead to drug resistance of sensitive bacteria and reduce the efficacy of drugs. Drug residues also have teratogenic, mutagenic, carcinogenic and hormonal effects. Antibiotics and their metabolites enter the environment through feces and urine, are enriched by animals and plants, enter the food chain, and endanger human health. Tetracycline antibiotics are widely used as feed drug additives to prevent intestinal infection and promote growth of aquatic products. There are as many as 23 kinds of antibiotics added in feed, of wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/413G01N27/30C08F222/14C08F212/08C08F2/44C08J9/26
Inventor 王富生何姝王小仙廖小卿
Owner 广东盈峰科技有限公司
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