71results about How to "High detection selectivity" patented technology

The invention provides a carboxylate radical bridged binuclear iron-sulfur-cluster fluorescent probe adopting the structure shown in the general formula I. A preparation method comprises steps as follows: anthracene methylamine and p-methoxycarbonylphenyl isocyanate have an addition reaction to produce substituted methyl benzoate; then corresponding carboxylic acid is obtained through hydrolysis; the obtained carboxylic acid and alkali react to produce carboxylate; the carboxylate is coordinated with a binuclear iron precursor to produce the fluorescent probe. Compared with the prior art, a carboxylate radical bridged binuclear iron-sulfur cluster with a metal complex close to nuclear structures of bio-enzymes is provided for the first time; the metal complex serves as the fluorescent probe, fluorine ion detection selectivity is good, fluorescence titration experiment operation is simple, and fluorescence change is sensitive.

An electrochemical sensor for sensing a gaseous analyte includes a substrate having at least two electrodes disposed thereon, and a carbon nanotube-polyaniline (CNT / PANI) film disposed on the substrate and in contact with at least two electrodes. The CNT / PANI film includes carbon nanotubes coated with a thin layer of polyaniline. The thickness of the polyaniline coating is such that electron transport can occur along and / or between the carbon nanotubes.

The invention discloses a method for constructing a photoelectrochemical sensor based on perovskite and black zirconium dioxide to detect 5-hydroxymethylcytosine deoxyribonucleotide (5hmC). The photoelectrochemical sensor includes an electrode, and the electrode is sequentially decorated with perovskite, mercaptopropionic acid, 5hmC and black ZrO 2 . First, a perovskite-based photoelectrochemical biosensor was constructed, using perovskite Bi 4 TaO 8 Good photoelectric activity and stability of Cl, M.HhaI methyltransferase catalyzes the specific reaction of hydroxymethyl groups with sulfhydryl groups on 5hmC, and black ZrO 2 Specific recognition of phosphate groups via black ZrO 2 with Bi 4 TaO 8 Cl forms a heterojunction to promote the transfer of photogenerated electrons to enhance the photoelectric signal, achieving high sensitivity and high specificity detection of 5hmC. The detection method of the invention is simple, easy to operate and low in cost, not only expands the application range of the two nanometer materials, but also realizes the detection of 5hmC.

The invention relates to a preparation method and application of a sensing chip for detecting atrazine. A single-molecule alkyl chain layer is formed on a gold film of the surface plasma resonance sensing chip; atrazine molecularly imprinted polymer reaction solution is prepared by modifying the single-molecule alkyl chain layer; after soaking and eluting, holes which only respond to atrazine molecules are left in a molecularly imprinted polymer film, and the sensing chip for detecting the atrazin is obtained; when the sensing chip is used for detecting a sample by using the surface plasma resonance technology, the resonance angle changes after the sensing chip adsorbs the atrazine molecules in the sample, and the resonance angle change value and the sample concentration are in directly proportional linear relationship, so that the atrazine in the sample can be quantitatively detected on the basis of the change of the resonance angle. According to the preparation method and the application, the surface plasma resonance technology and the molecular imprinting technology are adopted to prepare the sensing chip, and the prepared sensing chip used for detecting the atrazine has the characteristics of sensitivity, rapidness, accuracy and simplicity.