Preparation method and application of sensing chip for detecting atrazine

A sensor chip and atlafeng technology, which is applied in the field of sensor chip preparation, can solve the problems of complex and time-consuming detection of atlafeng, a large number of detection samples, and difficulty in carrying, etc., and achieves high sensitivity and sample size. Less, the effect of simple and convenient operation

Inactive Publication Date: 2014-02-12
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned detection method is complicated and time-consuming to detect Atla Feng, and the instrument is expensive and not easy to carry. limit

Method used

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  • Preparation method and application of sensing chip for detecting atrazine
  • Preparation method and application of sensing chip for detecting atrazine
  • Preparation method and application of sensing chip for detecting atrazine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) On the glass substrate, the gold film of 50nm is vacuum-evaporated to make a surface plasmon resonance sensor chip, and the amount of substance concentration that is put into the sensor chip is the ethanol of dodecyl mercaptan of 1mmol / L Soak in the solution for 24 hours, self-assemble monomolecular alkyl chain layer on the gold film, then take it out, rinse with ethanol, blow dry with nitrogen gas and set aside.

[0032] (2) Dissolve 0.1mmol of the template molecule Atla Feng and 0.3mmol of the functional monomer methacrylic acid (MAA) in 4ml of the porogen acetonitrile under the condition of avoiding light, mix them uniformly by ultrasonic mixing for 50min, and let stand for 1h; then add 0.4 mmol of crosslinking agent ethylene glycol dimethacrylate (EDGMA) and 0.08 mmol of initiator benzophenone were ultrasonically mixed for 4 minutes to mix uniformly, and then nitrogen gas was passed for 10 minutes to obtain a polymerization reaction liquid.

[0033] (3) Inject t...

Embodiment 2

[0041] (1) A 50nm gold film is vacuum-evaporated on a glass substrate to make a surface plasmon resonance sensor chip, and the sensor chip is put into a concentration of 1mmol / L dodecyl mercaptan and soaked in an ethanol solution After 48 hours, a monomolecular alkyl chain layer was self-assembled on the gold film, then removed, rinsed with ethanol, and dried with nitrogen gas for later use.

[0042] (2) Dissolve 0.1mmol of the template molecule Atla Feng and 0.5mmol of the functional monomer methacrylic acid in 4ml of the porogen acetonitrile under the condition of avoiding light, mix ultrasonically for 50min, and let it stand for 1h; then add 1.2mmol of the crosslinking agent Ethylene glycol dimethacrylate and 0.08 mmol initiator benzophenone were ultrasonically mixed for 4 minutes, and then nitrogen gas was passed for 10 minutes to obtain a polymerization reaction solution.

[0043] (3) Inject the polymerization reaction solution obtained in step (2) into the reaction tank,...

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Abstract

The invention relates to a preparation method and application of a sensing chip for detecting atrazine. A single-molecule alkyl chain layer is formed on a gold film of the surface plasma resonance sensing chip; atrazine molecularly imprinted polymer reaction solution is prepared by modifying the single-molecule alkyl chain layer; after soaking and eluting, holes which only respond to atrazine molecules are left in a molecularly imprinted polymer film, and the sensing chip for detecting the atrazin is obtained; when the sensing chip is used for detecting a sample by using the surface plasma resonance technology, the resonance angle changes after the sensing chip adsorbs the atrazine molecules in the sample, and the resonance angle change value and the sample concentration are in directly proportional linear relationship, so that the atrazine in the sample can be quantitatively detected on the basis of the change of the resonance angle. According to the preparation method and the application, the surface plasma resonance technology and the molecular imprinting technology are adopted to prepare the sensing chip, and the prepared sensing chip used for detecting the atrazine has the characteristics of sensitivity, rapidness, accuracy and simplicity.

Description

technical field [0001] The present invention relates to a preparation method and application of a sensor chip for detecting atlas, in particular, to a sensor chip based on surface plasmon resonance (Surface Plasmon Resonance, SPR) technology and molecular imprinting (Molecular imprinting) technology The invention relates to a preparation method of a sensor chip, and the sensor chip is used for detecting Atla Feng, belonging to the technical field of pesticide detection. Background technique [0002] Atrazine, common name in English: atrazine, chemical name: 2-chloro-4-diethylamino-6-isopropylamino-1,3,5-triazine, is a s-triazine herbicide It has the advantages of excellent herbicidal efficacy and low price, so it is widely used in countries all over the world. Since Atla Feng began to be used in our country, the consumption has increased every year. Although Atla Feng is a herbicide with low toxicity, it can interfere with the endocrine of animals and is a potential carcin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/55G01N21/552
Inventor 韦天新裴芳誉刘兰兰
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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