Electro-polymerization preparation method of electrochemical sensor for quickly detecting EGCG (Epigallocatechin-3-Gallate)

Abstract

The invention relates to an electro-polymerization preparation method of an electrochemical sensor for quickly detecting EGCG (Epigallocatechin-3-Gallate), and relates to a technology in the field of active ingredient analysis and detection of an agricultural product. The method mainly comprises the following steps of: pre-treating a glass-carbon electrode; performing electro-polymerization filming on the surface of the glass-carbon electrode; and eluting template molecules to obtain EGCG molecularly imprinted membrane modified electrode, wherein the sensor can be used for quick detection of an EGCG-containing actual sample in combination of an electrochemical working station. The electro-polymerization preparation method has the advantages of high selectivity, high operation efficiency, high stability of the sensor, capability of being recycled, low cost and suitability for quick, real-time and field detection, and is suitable for quick detection of the content of the main active ingredient EGCG in tea and products thereof and quality evaluation of the tea or the tea products.

Description

technical field

[0001] The invention relates to the technology in the field of analysis and detection of active components, in particular to an electrochemical sensor for rapid detection of EGCG and an electropolymerization preparation method thereof. Background technique

[0002] Epigallocatechin-3-gallate (EGCG) is mainly found in tea plants, with the highest content in green tea. It is the main component of green tea tea polyphenols and the main component of green tea catechins, accounting for About 50% of the total amount of catechins. EGCG has a wide range of biological activities, such as scavenging free radicals, anti-oxidation, anti-cancer, delaying aging, preventing cardiovascular diseases, anti-mutation, reducing fat and reducing weight, preventing radiation, enhancing oral health and other health care and pharmacological effects. main active ingredient. The quality and price of tea are determined by the content of EGCG to a certain extent. Therefore, it is of g...

Examples

Embodiment 1

[0023] The glassy carbon electrode was polished into a mirror surface with alumina particles of 0.3 μm and 0.05 μm in sequence, and the alumina powder adhering to the surface was rinsed off with distilled water, then ultrasonically washed in ultrapure water and absolute ethanol for 3 min, and cycled 3 times. After washing off the residue on the electrode surface, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer (pre-filled with nitrogen for 8 min to remove oxygen), and carried out cyclic voltammetry scanning in the voltage range of 0-0.8 V until

[0024] to obtain a stable cyclic voltammetry response. With glassy carbon electrode as working electrode, o-phenylenediamine ( o- PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.2) containing 5 mmol / L o...

Embodiment 2

[0029] The glassy carbon electrode was polished into a mirror surface with alumina particles of 0.3 μm and 0.05 μm in turn, and the alumina powder attached to the surface was rinsed with distilled water, followed by ultrasonic cleaning in ultrapure water and absolute ethanol for 5 min, and the cycle was repeated 6 times. Wash away residues from the electrode surface. Afterwards, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer solution (pre-filled with nitrogen for 10 min to remove oxygen), the cyclic voltammetry scan was performed in the voltage range of 0-0.8 V until a stable cyclic voltammetry response was obtained. With glassy carbon electrode as working electrode, o-phenylenediamine ( o -PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.5) con...

Embodiment 3

[0033] The glassy carbon electrode was polished to a mirror surface with 0.3 μm and 0.05 μm alumina suspension suede in turn, and after the alumina powder attached to the surface was rinsed off with distilled water, it was ultrasonically washed in ultrapure water and absolute ethanol for 5 min. Cycle 6 times to wash off the residue on the electrode surface. Afterwards, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer solution (pre-filled with nitrogen for 10 min to remove oxygen), the cyclic voltammetry scan was performed in the voltage range of 0-0.8 V until a stable cyclic voltammetry response was obtained. With glassy carbon electrode as working electrode, o-phenylenediamine ( o -PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.5) containing 10...

technical field

[0001] The invention relates to the technology in the field of analysis and detection of active components, in particular to an electrochemical sensor for rapid detection of EGCG and an electropolymerization preparation method thereof. Background technique

[0002] Epigallocatechin-3-gallate (EGCG) is mainly found in tea plants, with the highest content in green tea. It is the main component of green tea tea polyphenols and the main component of green tea catechins, accounting for About 50% of the total amount of catechins. EGCG has a wide range of biological activities, such as scavenging free radicals, anti-oxidation, anti-cancer, delaying aging, preventing cardiovascular diseases, anti-mutation, reducing fat and reducing weight, preventing radiation, enhancing oral health and other health care and pharmacological effects. main active ingredient. The quality and price of tea are determined by the content of EGCG to a certain extent. Therefore, it is of g...

Examples

Embodiment 1

[0023] The glassy carbon electrode was polished into a mirror surface with alumina particles of 0.3 μm and 0.05 μm in sequence, and the alumina powder adhering to the surface was rinsed off with distilled water, then ultrasonically washed in ultrapure water and absolute ethanol for 3 min, and cycled 3 times. After washing off the residue on the electrode surface, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer (pre-filled with nitrogen for 8 min to remove oxygen), and carried out cyclic voltammetry scanning in the voltage range of 0-0.8 V until

[0024] to obtain a stable cyclic voltammetry response. With glassy carbon electrode as working electrode, o-phenylenediamine ( o- PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.2) containing 5 mmol / L o...

Embodiment 2

[0029] The glassy carbon electrode was polished into a mirror surface with alumina particles of 0.3 μm and 0.05 μm in turn, and the alumina powder attached to the surface was rinsed with distilled water, followed by ultrasonic cleaning in ultrapure water and absolute ethanol for 5 min, and the cycle was repeated 6 times. Wash away residues from the electrode surface. Afterwards, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer solution (pre-filled with nitrogen for 10 min to remove oxygen), the cyclic voltammetry scan was performed in the voltage range of 0-0.8 V until a stable cyclic voltammetry response was obtained. With glassy carbon electrode as working electrode, o-phenylenediamine ( o -PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.5) con...

Embodiment 3

[0033] The glassy carbon electrode was polished to a mirror surface with 0.3 μm and 0.05 μm alumina suspension suede in turn, and after the alumina powder attached to the surface was rinsed off with distilled water, it was ultrasonically washed in ultrapure water and absolute ethanol for 5 min. Cycle 6 times to wash off the residue on the electrode surface. Afterwards, the electrode was electrochemically activated, that is, the electrode was placed in a solution containing 5 mmol / L K 3 [Fe(CN) 6 ] and 0.2 mol / L KCl in phosphate buffer solution (pre-filled with nitrogen for 10 min to remove oxygen), the cyclic voltammetry scan was performed in the voltage range of 0-0.8 V until a stable cyclic voltammetry response was obtained. With glassy carbon electrode as working electrode, o-phenylenediamine ( o -PD) is the functional monomer, and potassium chloride is the supporting electrolyte. The three-electrode system was placed in a phosphate buffer solution (pH 5.5) containing 10...

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