Nitrogen-oxide-doped carbon dot fluorescence probe for formaldehyde detection and preparation method of fluorescence probe

A technology of nitrogen-doped carbon dots and carbon dot fluorescence, applied in the field of detection, can solve the problems of complex detection methods and high cost of formaldehyde, and achieve the effects of low detection limit, convenient storage, and good water solubility

Active Publication Date: 2018-06-22
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a nitrogen oxide-doped carbon dot fluorescent probe for formaldehyde detection and a preparation method thereof, said nitrogen oxide-doped carbon dot for formaldehyde detection The point fluorescent probe and its preparation method should solve the technical problems of complex and high-cost methods for detecting formaldehyde in the prior art

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  • Nitrogen-oxide-doped carbon dot fluorescence probe for formaldehyde detection and preparation method of fluorescence probe
  • Nitrogen-oxide-doped carbon dot fluorescence probe for formaldehyde detection and preparation method of fluorescence probe
  • Nitrogen-oxide-doped carbon dot fluorescence probe for formaldehyde detection and preparation method of fluorescence probe

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Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Preparation of nitrogen oxide doped carbon dots

[0028] (1) Add 2.68g of citric acid monohydrate and 3.44g of urea into 40ml of deionized water, mix and stir to obtain a transparent solution;

[0029] (2) Transfer the solution in step (1) to a 50mL reactor, and react at 180°C for 12 hours to obtain a yellow nitrogen-doped carbon dot solution;

[0030] (3) Put the yellow solution in step (2) in an open beaker and stir for 2 days, and oxidize to obtain a blue nitrogen-doped carbon dot solution. Among them, with the extension of the oxidation time, the solution gradually changes from yellow to green, and finally appears blue, such as figure 1 Shown.

[0031] (4) Add ethanol to the oxidation solution in step (3), centrifuge to precipitate, repeatedly wash the precipitate with ethanol, and centrifuge to obtain a blue precipitate;

[0032] (5) Place the precipitate in step (4) in a blast drying oven for 0.5 hours, heating at 80°C to obtain a blue solid powder, which is wate...

Embodiment 2

[0036] Example 2 Performance analysis of nitrogen oxide-doped carbon dots for detecting formaldehyde

[0037] (1) Prepare 15 mL of a nitrogen oxide-doped carbon dot solution with a concentration of 0.01 mg / mL, select an excitation wavelength of 360 nm, and measure its fluorescence as a blank contrast value. Under vacuum sealing conditions, formaldehyde is injected into the solution, and the concentration of formaldehyde in the solution ranges from 0 to 300 μM. Select the excitation wavelength as 360nm, and measure the corresponding fluorescence record data;

[0038] (2) Repeat the experiment several times, draw the curve of the decrease of the fluorescence intensity of the carbon dot solution as the concentration of formaldehyde increases, and select the linear part for fitting to obtain the fitting equation I = −0.29 × C formaldehyde + 22.67 (R 2 = 0.99), the lowest detection limit is 4.5ppb, such as Image 6 Shown.

Embodiment 3

[0039] Example 3 Nitrogen oxide doped carbon dots are used for the detection of low-concentration formaldehyde

[0040] (1) Add a certain amount of formaldehyde to 15 mL of a nitrogen oxide doped carbon dot solution with a concentration of 0.01 mg / mL, so that the concentration of formaldehyde in the solution is 10 μmol / L. The excitation wavelength is selected as 360nm, and the fluorescence intensity of the measured solution drops to 19.66;

[0041] (2) Bring the fluorescence intensity value measured in step (1) into the fitting equation, and calculate the concentration of formaldehyde to be detected to be approximately 10.38 μmol / L;

[0042] (3) By analogy, add a certain amount of formaldehyde to 15mL of a nitrogen oxide doped carbon dot solution with a concentration of 0.01mg / mL, so that the concentration of formaldehyde in the solution is 15μmol / L, 20μmol / L, 30μmol / L and 40μmol / L. The excitation wavelength is selected as 360nm, and the measured fluorescence intensity drops to 18...

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Abstract

The invention provides a nitrogen-oxide-doped carbon dot fluorescence probe for formaldehyde detection. The fluorescence probe is synthesized from citric acid and urea as raw materials with a hydrothermal method, a prepared yellow carbon dot solution is oxidized into green or blue nitrogen-doped carbon dots, groups containing nitrogen and oxygen are located on surfaces of the nitrogen-doped carbondots, and average grain diameter of the nitrogen-doped carbon dots is smaller than 10 nm. The invention further provides a preparation method of the fluorescence probe. The fluorescence probe is synthesized from citric acid and urea as the raw materials with the hydrothermal method, the prepared nitrogen-doped carbon dot solution is yellow and is stirred for several hours to several days in an open beaker for oxidization, and a green or blue nitrogen-doped carbon dot solution is obtained. Fluorescence of the oxidized nitrogen-oxide-doped carbon dots is enhanced and fluorescence quenching occurs after the carbon dots act with formaldehyde, on the basis, an ultra-sensitive fluorescence method is established to be used for quantitative detection of formaldehyde, detection selectivity is good, and detection limit is low. The fluorescence probe can be stable in multiple metal ion solutions and has low cytotoxicity and good biocompatibility.

Description

Technical field [0001] The invention belongs to the field of detection science and relates to the detection of formaldehyde, in particular to a nitrogen oxide-doped carbon dot fluorescent probe for formaldehyde detection and a preparation method thereof. Background technique [0002] Formaldehyde is a colorless gas with a strong pungent odor and is easily soluble in water, alcohol and ether. Formaldehyde is harmful to the human body and highly toxic. After the human body is inhaled, it will destroy the body's proteins and enzymes, causing irreversible coagulation and necrosis of tissue cells. It has been listed as a known carcinogen and teratogen by the World Health Organization . The newly renovated rooms have high formaldehyde content, which is the main cause of many diseases. The detection and treatment of formaldehyde are urgent. The current formaldehyde detection methods generally include the following categories: fluorescence method, spectrophotometer, chromatography and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65G01N21/64B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C09K11/0883C09K11/65G01N21/643
Inventor 李慧珺孙熊王现英杨俊和
Owner UNIV OF SHANGHAI FOR SCI & TECH
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