Double-inhibition electrochemiluminescence sensor and method for measuring glyphosate

An electrochemistry and glyphosate technology, which is applied in the field of pesticide residue analysis and determination, can solve the problems of amperometry interference and high working potential, and achieve the effects of increasing luminous intensity, reducing production costs, and saving reagents

Inactive Publication Date: 2019-08-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use the amperometric method to detect H at +0.65V 2 o 2 , but due to the relatively high working potential

Method used

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  • Double-inhibition electrochemiluminescence sensor and method for measuring glyphosate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation method of Au-rGO / GCE modified electrode

[0039] (1) Preparation of graphene oxide: In a 500.0 mL three-necked flask, 6.8 g of potassium permanganate was dissolved in a mixed solution of 120.0 mL of concentrated sulfuric acid and 14.0 mL of phosphoric acid, and then 1.0 g of graphite powder was added and mixed evenly. The water bath continued to stir for 12 h; 140.0 mL of ice water was slowly added to the above solution, and then the unreacted potassium permanganate was oxidized with 30% hydrogen peroxide, and a brown-yellow mixture was observed. After the solution was cooled, it was ultrasonicated for more than 1.5 h. The solution was centrifuged and washed at 6000 rpm until neutral to obtain graphene oxide, and the graphene oxide (GO) was dried at 60°C to obtain solid graphene oxide;

[0040] (2) Preparation of graphene: Dilute the prepared GO to 0.5 mg / mL, take 20.0 mL into a three-necked flask, add 300.0 μL of ammonia water and 20.0 μL of hydra...

Embodiment 2

[0044] Example 2 Luminol-Au-L-cys-Cu 2+ Composite preparation method:

[0045] (1) Mix 1.0 mL, 15 mmol / L luminol and 5.0 mL, 0.25 mmol / L HAuCl 4 Mix and stir vigorously at room temperature for 12 h to obtain a luminol-Au NPs solution;

[0046] (2) At room temperature, add 100 μL, 6.0 mmol / L L-Cys aqueous solution to 1.0 mL luminol-Au NPs solution, and incubate at 4 °C for 5 h; continue to add 100.0 μL, 6 mmol / L Cu 2+ solution, and shake at room temperature for 5 min, luminol-Au-L-cys-Cu 2+ composite material.

Embodiment 3

[0047] Example 3 Preparation method of dual-suppression electrochemiluminescence sensor:

[0048] (1) Drop-coat 2.0 μL acetylcholinesterase and 10 μL choline oxidase onto the surface of Au-rGO modified glassy carbon electrode Au-rGO / GCE, and incubate overnight to obtain the modified electrode AChE-ChOx / Au-rGO / GCE;

[0049] (2) Add 8.0 μL of luminol-Au-L-cys-Cu dropwise on the electrode surface 2+ The composite material was cross-linked by incubation with glutaraldehyde at 4°C for 2 h to obtain luminol-Au-L-cys-Cu 2+ / AChE-ChOx / Au-rGO / GCE;

[0050] After incubation in the above steps, gently rinse with PBS pH 7.4 to remove physically adsorbed material.

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Abstract

The invention relates to the technical field of analysis and determination of agricultural residues, in particular to a double-inhibition electrochemiluminescence sensor and a method for measuring glyphosate by using the same, and the method comprises the steps of preparing a double-inhibition sensor, an operation method for measuring glyphosate by using the sensor and the like.. The double-inhibition sensor utilizes the enzymatic reaction and the catalysis of copper complex peroxidase, and the synergistic effect is generated by combining two signal conversion modes, so that the detection sensitivity of the sensor is effectively improved; and the method for measuring glyphosate by using the double-inhibition electrochemiluminescence sensor is used for detecting glyphosate, and has the advantages of small interference of coexisting substances, good selectivity and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of analysis and determination of pesticide residues, in particular to a dual-inhibition electrochemiluminescence sensor and a method for using it to determine glyphosate, including the preparation steps of the dual-inhibition sensor and the operation method for using the sensor to determine glyphosate, etc. ; This dual-inhibition sensor utilizes the enzymatic reaction and the copper complex peroxidase catalysis, which is a combination of two signal conversion methods to produce a synergistic effect; glyphosate inhibits the enzymatic reaction and simultaneously inhibits the peroxidase-like Oxidase catalysis, highly sensitive determination of glyphosate, less interference from coexisting substances, good selectivity. Background technique [0002] The development of pesticide residue detection technology, especially rapid detection technology, and timely monitoring of pesticide residues in the environment and f...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/30G01N27/48
CPCG01N21/76G01N27/308G01N27/48
Inventor 周长利刘汉彪陈培培刘建辉衣姜乐夏方诠
Owner UNIV OF JINAN
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