A kind of nanometer material that detects dimethoate and preparation method thereof

Abstract

The invention aims at providing a nano material for detecting dimethoate. The nano material comprises the following components by weight percent: 97.43 to 99.31 percent of sodium silicate, 0.06 to 0.23 percent of TPPS4 and 0.63 to 2.34 percent of plant-esterase. The plant-esterase is fixed by adopting a sol-gel technology, the fixation process is a physical embedding process, a gel network is gradually formed surrounding the enzyme, the structure completeness and isotropy and biological activity of surface microstructure of the enzyme can be maintained by virtue of the moderate reaction condition, amorphous structure of the gel and biological compatibility. Then the TPPS4 enters the gel network by virtue of the micropores on the surface of the gel to be reacted with the plant-esterase so as to form aggregation on the surface of the enzyme. In the gel network, the enzyme and the aggregation have less possibility of being subjected to the unfavorable influence of unfavorable environmental factors, but also the structure is more stable, the precipitate is unlikely to form, the enzyme and the aggregation are easy to store, the reaction activity can be well protected, and the detection reaction is favored.

Description

technical field [0001] The invention belongs to the technical field of materials for detecting organophosphorus pesticides and the preparation thereof, in particular to a nanometer material for detecting dimethoate and a preparation method thereof. Background technique [0002] Dimethoate is an organophosphorus systemic insecticide with contact and stomach poisoning, its chemical name is O, O-dimethyl-S-(N-methylcarbamoylmethyl) dimethoate Phosphorothioate. Dimethoate is an insecticide with a wide range of uses and a large yield. It has a good control effect on piercing-sucking mouthparts pests, such as aphids and whiteflies. However, dimethoate is volatile, and a large amount of lost pesticides volatilize into the air, water body or settle and gather in the soil to pollute agricultural, livestock and fishery by-products, and transfer to the human body through the enrichment of the food chain, causing harm to the human body. Studies have shown that dimethoate has potential...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of nanometer material and preparation method thereof that detects dimethoate for the deficiencies of existing biological enzyme detection materials, have production cost low, can realize the rapid, selective detection of dimethoate, and detect The process does not require other reagents other than the sample, etc.

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