A method for quantitatively detecting l-cysteine
A technology for quantitative detection of cysteine, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of miniaturization and high technical requirements of electrochemical workstations, achieve low cost, improve sensitivity, good specific effect
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Embodiment 1
[0018] Construction of Glucose / Oxygen Biofuel Cell
[0019] 1. Preparation of Bioanode
[0020] Mix 4 μL of multi-walled carbon nanotubes (2 mg / mL), 5 μL of vitamin K 3 and 10 μL of FAD-GDH solution (5 mg / mL) were sequentially drip-coated on the glassy carbon electrode, and after drying, 5 μL of Nafion solution (0.05 wt%) was added dropwise. Dry at room temperature to prepare a FAD-GDH-based bioanode.
[0021] 2. Preparation of Biocathode
[0022] 4 μL of multi-walled carbon nanotubes (2 mg / mL) and 10 μL of laccase solution (5 mg / mL) were successively drop-coated on the glassy carbon electrode and dried. Then 2 μL of glutaraldehyde solution (2 wt%) was added dropwise and dried at 4°C to prepare a laccase-based biocathode.
[0023] Insert bioanode and biocathode into 5mL acetic acid-sodium acetate buffer solution (pH 5.5) to assemble biofuel cell, 30mM glucose as fuel, and add 2mM ABTS as electron mediator, use CHI660E electrochemical workstation, adopt linear sweep voltamm...
Embodiment 2
[0025] Utilizing the constructed glucose / oxygen biofuel cell, in Cu 2+ Highly sensitive detection of L-cysteine is achieved in the presence of:
[0026] Add 4μM of Cu to the battery of the above example 2+ , and then use a multimeter to detect the open circuit potential of the battery. Then add different concentrations of L-cysteine and record the corresponding open circuit potential value. Finally, take the concentration of L-cysteine as the abscissa, and the variation of the open circuit potential as the ordinate, draw a standard curve, a straight line can be obtained, and the slope can be obtained, which is the slope of the working curve of L-cysteine. The linear relationship between the change of potential and the concentration of L-cysteine (see image 3 ).
[0027] The working curve of L-cysteine is as follows figure 2 As shown, it can be seen from the figure that the R of the working curve 2 The value reaches 0.997, and the linear fit is very good.
[...
Embodiment 3
[0030] Determination of L-cysteine content in a local brand L-cysteine compound tablet:
[0031] 1. Sample treatment: Take an appropriate amount of L-cysteine compound tablets of a certain local brand, grind and dissolve in ultrapure water, filter with a 0.22 μm disposable filter membrane, and use 100mM acetic acid-sodium acetate buffer ( pH 5.5) was diluted to volume.
[0032] 2. The detection process of L-cysteine: Assemble the fuel cell with 5mL acetic acid-sodium acetate buffer solution (pH 5.5) containing 30mM glucose and 2mM ABTS as the base solution, and add 4mM Cu to the base solution 2+ The measured open circuit potential OCP of the system 1 , and then add different volumes of L-cysteine samples to the bottom solution successively, and measure the open circuit potential OCP 2 .
[0033] 3. Calculation of L-cysteine concentration: take the change of open circuit potential (OCP 2 –OCP 1 ) within the range of the standard curve to calculate the dilution ra...
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