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Enzyme immobilization matrix prepared by using mesoporous-silica-coated gold nanorod(AuNR@mSiO2) as dopant and biological detection application thereof

A technology of silicon dioxide and gold nanorods, which is used in measurement devices, material analysis by electromagnetic means, instruments, etc., to achieve the effects of good specificity, good application prospects and good stability

Active Publication Date: 2019-04-02
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Mesoporous silica-coated gold nanorods (i.e., AuNR@mSiO 2 ) used in the preparation of enzyme-immobilized substrates

Method used

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  • Enzyme immobilization matrix prepared by using mesoporous-silica-coated gold nanorod(AuNR@mSiO2) as dopant and biological detection application thereof
  • Enzyme immobilization matrix prepared by using mesoporous-silica-coated gold nanorod(AuNR@mSiO2) as dopant and biological detection application thereof
  • Enzyme immobilization matrix prepared by using mesoporous-silica-coated gold nanorod(AuNR@mSiO2) as dopant and biological detection application thereof

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Embodiment 1

[0035] I. Doping AuNR@mSiO in ‘TiO2-Chitosan’ Hybrid Hydrogel 2 And modified glassy carbon electrode

[0036] a. Add 50μL 0.6nM AuNR@mSiO 2 aqueous solution and 450 μL of pre-synthesized TiO 2 The hydrogel precursor liquid was mixed rapidly, and then 5 μL of 0.5% chitosan was added to the mixed liquid and mixed rapidly.

[0037] b. Take 4 μL of the above mixture and add it dropwise on the glassy carbon electrode, and form ‘AuNR@mSiO in a few minutes in the air 2 - Titanium dioxide-chitosan’ nanocomposite hydrogel.

[0038] II. Preparation of acetylcholinesterase electrode and detection of organophosphate pesticides

[0039] a. First, the above ‘AuNR@mSiO 2 - Titanium dioxide-chitosan' nanocomposite hydrogel modified glassy carbon electrode was immersed in 5mL 0.2% chitosan solution, and a negative potential of -2.5V was applied on the electrode (relative to the silver / silver chloride / 3M KCl reference electrode ) for 20 seconds, electrodeposit chitosan film on the electro...

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Abstract

The invention, which relates to the technical field of composite material preparation and bio-detection products, discloses an enzyme immobilization matrix prepared by using a mesoporous-silica-coatedgold nanorod(AuNR@mSiO2) as a dopant and application thereof to an immobilized enzyme and in biological detection. A AuNR@mSiO2 solution is mixed with titanium dioxide hydrogel precursor liquid and then the mixed solution is mixed with a chitosan solution, and coating of the final mixed solution is carried out to obtain AuNR@mSiO2-doped 'titanium dioxide-chitosan' enzyme immobilization matrix. The acetylcholin esterase electrode with AuNR@mSiO2 doped has the substantially improved detection sensitivity to the acetylcholin esterase inhibitor being an organophosphorus insecticide. The doping process is simple in operation; the prepared enzyme immobilization matrix and enzyme electrode product have high usage stability and storage stability; the enzyme substrate and enzyme inhibitor detection sensitivity is high; and the precision and the recovery rate are high; and thus the enzyme immobilization matrix has the broad application prospects.

Description

technical field [0001] The invention relates to the technical field of composite material preparation and biological detection products, and more specifically relates to doping the gold nanorod coated with silicon dioxide into the enzyme immobilization matrix, and applying the enzyme immobilization matrix to the immobilized enzyme and carrying out biological detection applications. Background technique [0002] The specific recognition and catalysis of enzymes to substrates has a wide range of applications in biological detection, biosensors, and bioengineering. The enzyme immobilization technology can bind or limit the enzyme in a certain area, maintain the unique catalytic reaction of the enzyme, improve the tolerance of the enzyme to changes in temperature and pH, improve storage stability, and recover and repeat the enzyme. Therefore, resources and energy can be saved, and ecological environment pollution can be reduced or prevented. In biodetection and biosensor appli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 崔惠芳张亭亭武文文
Owner ZHENGZHOU UNIV
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