Rapid colorimetric detection method for acetamiprid based on nano-enzyme catalysis

Abstract

The invention discloses a rapid colorimetric detection method for acetamiprid based on nano-enzyme catalysis. The rapid colorimetric detection method comprises the following steps: A, drawing a standard curve for the variation of the absorbency of acetamiprid; B, based on the standard curve for the variation of the absorbency of acetamiprid, establishing a linear regression equation for the relationship between the concentrations of acetamiprid and the absorbency of acetamiprid; C, preparing a to-be-detected solution C with an unknown acetamiprid concentration; and D, subjecting the to-be-detected solution C to absorption spectrum scanning so as to measure the absorbency of acetamiprid at a wavelength of 735 nm, calculating a difference between the absorbency of the to-be-detected solution C and the absorbency of an A2 product, and substituting the difference into the linear regression equation so as to obtain the concentration of acetamiprid in the to-be-detected solution C. The method does not require professional operations, and has the advantages of simple operation, rapid detection, low cost, intuitive test results, suitability for on-site detection, etc.

Description

technical field [0001] The invention relates to a method for detecting acetamiprid, in particular to a rapid colorimetric detection method for acetamiprid based on nanozyme catalysis. Background technique [0002] Acetamiprid is a new type of insecticide, which has contact killing, stomach toxicity and strong osmotic effect, quick insecticidal effect, low dosage, high activity, wide insecticidal spectrum, long duration, and good environmental compatibility Wait. Acetamiprid can be used to control pests of rice, vegetables, fruit trees, tea trees such as Hemiptera, Lepidoptera and Coleoptera, and can also kill eggs, so it is widely used in pest control of agricultural products and meat products. According to my country's pesticide toxicity classification standards, acetamiprid is a moderately toxic insecticide. Due to the widespread use of acetamiprid, it has entered and widely exists in the human living environment, posing a great potential threat to human health and livin...

AI Technical Summary

Problems solved by technology

Instrumental analysis methods mainly include gas chromatography (GC), high performance liquid chromatography (HPLC), ion chromatography and chromatography-mass spectrometry, etc. The disadvantage of instrumental analysis methods is that the required equipment is expensive and requires professionals operation, not suitable for rapid detection in the field

The disadvantage of the enzyme inhibition method is that its sensitivity and reproducibility need to be improved, and the sensitivity is not as good as that of the instrument method

The disadvantage of immunoassays is that they are prone to false positives

The disadvantage of the biosensor method is that the stability of many biosensor technologies is not high, resulting in problems such as short service life in practice.

The disadvantage of chemiluminescence technology is that its signal output is easily disturbed by the environment, resulting in unreliable detection results

Traditional colorimetry includes visual colorimetry and photoelectric colorimetry, wherein the standard series of methods are commonly used in visual colorimetry, but the disadvantage is that there are subjective errors in eye observation, and the accuracy is relatively low; photoelectric colorimetry is compared to Although visual colorimetry eliminates subjective errors, the measurement accuracy and sensitivity are still low, and the application range is narrow

In addition, the enzyme inhibition method used in recent years for the colorimetric detection of acetamiprid pesticide residues has low sensitivity and high cost.

[0004] Most of the existing colorimetric detection methods using gold nanoparticles use its aggregation and discoloration effect. The disadvantage is that this method is easily affected by non-specific aggregation caused by non-targets. Non-specific aggregation will lead to inaccurate results and insufficient specificity.

Therefore, the existing detection methods have the disadvantages of requiring professionals to operate, complex operation, long detection time, high cost, unintuitive detection results, and not suitable for on-site rapid detection.

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