Acetamiprid pesticide detection method based on carbon dot fluorescence inner-filter effect

Abstract

The invention discloses an acetamiprid pesticide detection method based on a carbon dot fluorescence inner-filter effect. The detection method comprises the following steps: A1, preparing a to-be-detected sample solution of a known acetamiprid concentration so as to obtain a product A1; A2, preparing a blank control solution to prepare a product A2; A3, respectively scanning the product A1 and theproduct A2 by a fluorophotometer to determine the corresponding fluorescence intensity of an emission peak of the solution at 437nm so as to obtain a fluorescence emission spectrum; A4, drawing to obtain a standard curve between the acetamiprid concentration and the fluorescence intensity change according to the fluorescence emission spectrum; B, establishing a regression equation relates to a mutual relation between the acetamiprid concentration and the fluorescence intensity according to the standard curve; C, preparing a to-be-detected solution of an unknown acetamiprid concentration so asto obtain a product C; and D, scanning the product C by the fluorophotometer to determine the corresponding fluorescence intensity of the emission peak of the solution at 437nm, calculating the difference of the fluorescence intensity of the product C and the product A2, and substituting the difference of the fluorescence intensity into an equation of linear regression, thereby obtaining the concentration of the acetamiprid in the product C. The method has the advantages of being convenient in construction, high in sensitivity, excellent in specificity and the like.

Description

technical field [0001] The present invention relates to a method for detecting acetamiprid pesticide, in particular to a method for detecting acetamiprid pesticide based on the inner filter effect of carbon dot fluorescence. Background technique [0002] Acetamiprid (N-(N-cyano-ethylimino)-N-methyl-2-chloropyridine-5-methylamine) is a new type of insecticide that acts on the synapses of the insect nervous system. The nicotinic acetylcholine receptors interfere with the stimulation of the insect nervous system, causing blockage of the nervous system pathways, resulting in the accumulation of the neurotransmitter acetylcholine at the synapse, resulting in paralysis and eventual death of the insect. Acetamiprid is used to control pests of rice, vegetables, fruit trees, tea trees and other Ptera and Coleoptera. It is widely used because of its high activity, low dosage, fast insecticidal effect, wide insecticidal spectrum and long duration of effect. Pest control for agricultur...

AI Technical Summary

Problems solved by technology

Instrumental analysis methods mainly include high performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), etc. The disadvantage of instrumental analysis methods is that the required instruments and equipment are expensive and require Professional operation, not suitable for quick detection on site

The disadvantage of the enzyme inhibition method is that its sensitivity and reproducibility need to be improved, and the sensitivity is not as good as that of the instrument method

The disadvantage of immunoassays is that they are prone to false positives

The disadvantage of colorimetry is that there are subjective errors in eye observation, and the accuracy is relatively low

The vast majority of colorimetric detection methods using gold nanoparticles use its aggregation and discoloration effect, which is susceptible to non-specific aggregation caused by non-targets. The effect is easily disturbed by the environment

The disadvantage of chemiluminescence technology is that its signal output is easily disturbed by the environment, resulting in unreliable detection results

Although the electrochemical method has high sensitivity and specificity, it usually requires modification of the electrode and some complex instruments such as an electrochemical workstation.

At present, the fluorescence method mainly uses heavy metal quantum dots and organic dyes as the fluorescence source, which is harmful to the environment and human body.

Images