Method for rapidly identifying and detecting tropomyosin based on intermediate infrared spectrum

A tropomyosin, infrared spectroscopy technology, applied in the field of rapid identification of allergen myosin based on mid-infrared spectroscopy, rapid identification and detection of allergen myosin in crustaceans and shellfish, to reduce The effect of cost and operation difficulty, strong universality, and simple processing method

Inactive Publication Date: 2018-12-14
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In existing studies, infrared detection and analysis are used to study the secondary structure of proteins, but there is no report on the detection or identification of proteins using infrared spectroscopy

Method used

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  • Method for rapidly identifying and detecting tropomyosin based on intermediate infrared spectrum
  • Method for rapidly identifying and detecting tropomyosin based on intermediate infrared spectrum
  • Method for rapidly identifying and detecting tropomyosin based on intermediate infrared spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The aquatic product samples of Penaeus vannamei were processed to extract tropomyosin.

[0042] The pretreatment method for aquatic products is specifically to remove the shells and heads of the shrimp and remove the shrimp line, extract the shrimp meat with acetone until it is colorless, and then add 20mM Tris-HCl buffer solution (containing 0.5mol / L NaCl, pH 9.2), homogenate for 30s to obtain muscle homogenate; centrifuge at 10000r / min low temperature for 20min to obtain crude tropomyosin extract.

[0043] The tropomyosin in the crude tropomyosin extract is enriched by conventional experimental methods such as ammonium sulfate precipitation and isoelectric point precipitation, and the allergen is purified by conventional desalting means, namely dialysis, and then purified The final tropomyosin was freeze-dried to obtain tropomyosin (Tm) freeze-dried powder.

[0044] Using Spectrum GX Fourier Transform Infrared Spectrometer (Perkin Elmer Company), and solid measuremen...

Embodiment 2

[0052] Get Penaeus vannamei, grass prawns, base prawns, prawns, pipi shrimps, stone crabs, swimming crabs, clams and razor clams respectively, use the method described in Example 1 to extract its tropomyosin freeze-dried powder, and use the method of Example 1 Perform mid-infrared spectroscopy data collection.

[0053] Infrared absorption spectra of tropomyosin in different shrimp, shellfish and crab aquatic products (1800-1000cm -1 )Such as image 3 and Figure 5 shown.

[0054] Depend on image 3 and Figure 4 It can be seen that the amide I bands of the tropomyosin maps in each aquatic product are all at 1638-1647cm -1 Within the range; the amide II bands are all at 1536-1543cm -1 within range.

[0055] Depend on Figure 5 and Figure 6 It can be seen that the amide Ⅰ band and amide Ⅱ band of the tropomyosin map of stone crab and portunus crab are also within this range.

[0056] The second-order derivative is further carried out on the obtained infrared spectrum,...

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Abstract

The invention belongs to the field of detection, and provides a method for rapidly identifying and detecting a main allergen, tropomyosin, in an aquatic product based on an intermediate infrared spectrum technology. By obtaining an intermediate infrared spectrogram of a detected sample, through second-order derivation, the infrared spectrogram characteristic peak of the tropomyosin and the infrared spectrogram of the detected sample are compared, and thus rapid qualitative identification and quantitative detection of the tropomyosin are achieved. The tropomyosin can be rapidly detected withoutantibodies, the purposes of rapid qualitative identification and quantitative detection can further be achieved, and meanwhile the requirement of non-destructive food is met.

Description

technical field [0001] The invention belongs to the field of biological identification, and relates to an identification and detection method of the main allergen myosin in aquatic products, in particular to a rapid identification method of the allergen myosin based on mid-infrared spectrum technology, which is used for rapid identification and detection of crustaceans and the allergen myosin in shellfish. Background technique [0002] Among aquatic products, shrimp, crab (crustaceous) and shellfish are the main allergenic foods of aquatic products, and tropomyosin (Tm) is the most important allergen, and the allergic symptoms caused by it are becoming more and more complicated and severe; However, traditional cooking methods cannot degrade tropomyosin, so such aquatic products often cause food allergy events, so it is very necessary to explore a method for rapid identification and detection of allergen myosin. [0003] The current allergen myosin detection technology mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563
CPCG01N21/3563
Inventor 卢瑛李娅茹许长华
Owner SHANGHAI OCEAN UNIV
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