Application of Enzyme Immobilization Matrix in the Field of Biological Detection of Organophosphorus Pesticides

Abstract

The invention, which relates to the technical field of composite material preparation and bio-detection products, discloses an enzyme immobilization matrix prepared by using a mesoporous-silica-coatedgold nanorod(AuNR@mSiO2) as a dopant and application thereof to an immobilized enzyme and in biological detection. A AuNR@mSiO2 solution is mixed with titanium dioxide hydrogel precursor liquid and then the mixed solution is mixed with a chitosan solution, and coating of the final mixed solution is carried out to obtain AuNR@mSiO2-doped 'titanium dioxide-chitosan' enzyme immobilization matrix. The acetylcholin esterase electrode with AuNR@mSiO2 doped has the substantially improved detection sensitivity to the acetylcholin esterase inhibitor being an organophosphorus insecticide. The doping process is simple in operation; the prepared enzyme immobilization matrix and enzyme electrode product have high usage stability and storage stability; the enzyme substrate and enzyme inhibitor detection sensitivity is high; and the precision and the recovery rate are high; and thus the enzyme immobilization matrix has the broad application prospects.

Description

technical field

[0001] The invention relates to the technical field of composite material preparation and biological detection products, and more specifically relates to doping the gold nanorod coated with silicon dioxide into the enzyme immobilization matrix, and applying the enzyme immobilization matrix to the immobilized enzyme and carrying out biological detection applications. Background technique

[0002] The specific recognition and catalysis of enzymes to substrates has a wide range of applications in biological detection, biosensors, and bioengineering. The enzyme immobilization technology can bind or limit the enzyme in a certain area, maintain the unique catalytic reaction of the enzyme, improve the tolerance of the enzyme to changes in temperature and pH, improve storage stability, and recover and repeat the enzyme. Therefore, resources and energy can be saved, and ecological environment pollution can be reduced or prevented. In biodetection and biosensor appli...

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