Application of Enzyme Immobilization Matrix in the Field of Biological Detection of Organophosphorus Pesticides
A technology for biological detection and pesticides, which is used in measuring devices, instruments, scientific instruments, etc., to achieve the effects of good specificity, good application prospects and good stability
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[0035] I. Doping AuNR@mSiO in ‘TiO2-Chitosan’ Hybrid Hydrogel 2 And modified glassy carbon electrode
[0036] a. Add 50μL 0.6nM AuNR@mSiO 2 aqueous solution and 450 μL of pre-synthesized TiO 2 The hydrogel precursor liquid was mixed rapidly, and then 5 μL of 0.5% chitosan was added to the mixed liquid and mixed rapidly.
[0037] b. Take 4 μL of the above mixture and add it dropwise on the glassy carbon electrode, and form ‘AuNR@mSiO in a few minutes in the air 2 - Titanium dioxide-chitosan’ nanocomposite hydrogel.
[0038] II. Preparation of acetylcholinesterase electrode and detection of organophosphate pesticides
[0039] a. First, the above ‘AuNR@mSiO 2 - Titanium dioxide-chitosan' nanocomposite hydrogel modified glassy carbon electrode was immersed in 5mL 0.2% chitosan solution, and a negative potential of -2.5V was applied on the electrode (relative to the silver / silver chloride / 3M KCl reference electrode ) for 20 seconds, electrodeposit chitosan film on the electro...
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