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Fluorescent colorimetric chemical-sensitive material as well as synthesis method and application thereof

A chemically sensitive, synthetic method technology, applied in the field of fluorescent colorimetric chemically sensitive materials and their synthesis, can solve the problems of poor solubility of the host compound, long response time, and high detection limit, and achieves short response time, simple synthesis method, and good choice. sexual effect

Active Publication Date: 2012-07-04
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, small molecule fluorescent sensitive materials for highly sensitive detection of lipopolysaccharides have rarely been reported.
Existing methods for detecting lipopolysaccharides have some problems in practical application, such as the interference of various competing anions on the determination, poor solubility of the main compound in water, long response time, high detection limit, and expensive instruments and many more

Method used

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  • Fluorescent colorimetric chemical-sensitive material as well as synthesis method and application thereof
  • Fluorescent colorimetric chemical-sensitive material as well as synthesis method and application thereof
  • Fluorescent colorimetric chemical-sensitive material as well as synthesis method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A fluorescent colorimetric chemically sensitive material and its synthesis method and application:

[0069] Put 1 mole of compound T1-A, 2.5 moles of compound T2-A and 14 moles of anhydrous ferric chloride in a 100ml two-necked flask, add 50ml of anhydrous chloroform under nitrogen protection, and stir at room temperature After 48 hours, the solvent was dried under reduced pressure, a large amount of methanol was added to wash the precipitate and dried to obtain solid I. Gel permeation chromatography test molecular weight: 1.061e+5.

[0070] The molecular formulas of T1-A and T2-A are respectively:

[0071]

[0072] The compound I prepared in Example 1 was used as a fluorescent colorimetric chemically sensitive material to evaluate the response of the compound to lipopolysaccharide.

[0073] Add 25 μl, 0.2 mM LPS to 2 ml of 50 μM HEPES buffer solution of the compound I, such as figure 1 As shown in , the color of compound I solution changed from yellow to red befo...

Embodiment 2

[0080] A method for synthesizing a fluorescent colorimetric chemically sensitive material, comprising the following steps:

[0081] Put 1 mole of compound T1-A, 10 moles of compound T2-A and 44 moles of anhydrous ferric chloride in a 100ml two-necked flask, add 50ml of anhydrous chloroform under nitrogen protection, and stir at room temperature After 48 hours, the solvent was dried under reduced pressure, and a large amount of methanol was added to wash the precipitate and dried. Solid II was obtained. Gel permeation chromatography test molecular weight: 1.16e+5. The molecular formulas of T1-A and T2-A are the same as in Example 1, respectively.

[0082] The same concentration and the same amount of bacterial cultures of Proteus, Shigella, Streptococcus, and Dipneumococcus were added to the obtained saline solution of Compound II, and the changes in the ultraviolet absorption spectrum of Compound II before and after the addition of bacteria were observed. The spectra of Pro...

Embodiment 3

[0084] A method for synthesizing a fluorescent colorimetric chemically sensitive material, comprising the following steps:

[0085] Put 1 mole of compound T1-A, 40 moles of compound T2-A and 164 moles of anhydrous ferric chloride in a 100ml two-necked flask, add 50ml of anhydrous chloroform under nitrogen protection, and stir at room temperature After 48 hours, the solvent was dried under reduced pressure, and a large amount of methanol was added to wash the precipitate and dried. Solid III was obtained. Gel permeation chromatography test molecular weight: 1.33e+5.

[0086]The same concentration and the same amount of bacterial culture solution of Brucella, Legionella pneumophila, Bacillus anthracis, and Bacillus diphtheria were added respectively to the methanol and ethanol mixed organic solvent solution of compound III obtained, and the ultraviolet absorption spectrum of compound III was observed before and after adding bacteria It was found that the spectra of Brucella an...

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Abstract

The invention discloses a fluorescent colorimetric chemical-sensitive material as well as a synthesis method and application of the material. The material has the following structural formula shown in the specification, wherein R<1>, R<2>, R><3>, R<4>, R<5>, R<6>, R<7>, R<8> and R<9> are selected from hydrogen atom, C1-18 alkyl, aryl, ester group or ether group; Ar is selected from thiophene, pyrrole, benzene, naphthalene, anthracene, pyrene, indole, coumarin, fluorescein, carbazole, rhodamine, cyano dye, fluorene or quinoline; m is 1-1000; n is 1-1000; and X<-> is selected F<->, Cl<->, Br<->, or I<->. The compound can be used for detecting existence and content of lipopolysaccharide.

Description

technical field [0001] The invention belongs to the fields of organic chemistry and analytical chemistry, and in particular relates to a fluorescent colorimetric chemically sensitive material and its synthesis method and application. Background technique [0002] Lipopolysaccharide (lipopolysaccharide) is a large molecule composed of lipids and polysaccharides linked by covalent bonds. Lipopolysaccharide is released when Gram-negative bacteria such as E. coli and Salmonella enterica multiply or lyse. It is a major component of the outer membrane of Gram-negative bacteria and is a potent bacterial toxin known as endotoxin. [0003] Gram staining is a differential staining method widely used in bacteriology, founded in 1884 by the Danish physician Gram. Bacteria are dyed with basic dye crystals, and then mordant with iodine solution, and then decolorized with alcohol. Under certain conditions, some bacteria will not be decolorized, and some can be decolorized. Therefore, bac...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08G61/12G01N21/78G01N21/64
Inventor 汪鹏飞蓝敏焕刘卫敏吴加胜葛介超张文军
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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