Culture technology for tissues of baeckea frutescens
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A technology of tissue culture and pinus pine, which is applied in the field of plant tissue culture, can solve the problems of differences in medicinal ingredients, unfavorable promotion of good varieties of pinus pinus, and low germination rate of seeds, etc.
Inactive Publication Date: 2018-01-16
罗荣棋
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Problems solved by technology
In the past, Pinus pine seedlings were mainly propagated by seed sowing. Due to the low germination rate of the seeds, it was far from meeting the needs of large-scale production of Pinus pine seedlings.
Moreover, the differences in medicinal components between the offspring of seed propagation are unfavorable for the popularization of excellent varieties of Pinus pine
Method used
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Embodiment 1
[0015] (1) Bud induction culture: Take the stem section of Pinus pine with buds as explants, cut off the leaves, cut into 6.0 long segments with 4 axillary buds, soak them in washing powder solution for 6 minutes, and then gently brush them with a soft brush. Lightly brush the surface of the explant to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 6 seconds, then wash it with sterile water for 4 times, and then use 0.2% L Mercury solution was sterilized for 12 minutes, rinsed with sterile water for 4 times, and then wiped dry the water drops on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Illuminate 12 hours every day, cultivate 32 days under the condition of light intensity 1600lx, count its bud induction rate and growth situation, the bud induction rate is 88%. The bud induction medium is: ...
Embodiment 2
[0020] (1) Bud induction culture: Take the stem section of Pinus pine with buds as explants, cut off the leaves, cut into 6.5-long segments with 5 axillary buds, soak them in washing powder solution for 15 minutes, and then gently brush them with a soft brush. Lightly scrub the surface of the explant to remove the dust and part of the bacteria on the surface, then rinse it with tap water for 2 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 18 seconds, then wash it with sterile water for 8 times, and then use 0.1% L Mercury solution was sterilized for 20 minutes, rinsed with sterile water for 6 times, and then wiped dry the water droplets on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Illuminate 15 hours every day, cultivate 35 days under the condition of light intensity 2000lx, count its bud induction rate and growth situation, the bud induction rate is 84%. The bud induction...
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Abstract
The invention discloses a culture technology for tissues of baeckea frutescens. The baeckea frutescens is also named as broom twigs, chicken-shaped pine, saokamu, abies holophylla and litsea viridis.The baeckea frutescens belongs to short and small shrubs, is rare dungarunga, and is 1-2m in height; the baeckea frutescens includes more bald branches; the baeckea frutescens is simple-leaf, the leaves are opposite, and are upright or expanded; leaves are linear and conical, tips are sharp, grooves are formed on the leaves, bulges are formed under the leaves, and the leaves contain transparent glandular opaque spots; flowers are small and white, singly grow at the axils, each base has 2 bracteoles and a short handle; the porocalyx is bell-shaped, includes 5 cracks, and is membranous and persistent; and 5 petals are round, the ovary has 3 locules and each locule contains a plurality of ovules. Capsules are about 1mm in length and are cracked at the upper parts. The seeds are cornered. Thebaeckea frutescens flower in July and August. The whole herb or root of the baeckea frutescens can be used into medicines, can be picked in the whole year and can be dried for use or freshly used. Theculture technology disclosed by the invention adopts stem sections with buds of the baeckea frutescens as explants, realizes in-vitro regeneration of the baeckea frutescens by the processes of bud induction, bud proliferation culture, rooting culture, seedling hardening and transplantation and the like, and provides technical guarantee for massive breeding, rapid propagation and new-variety promotion of the baeckea frutescens.
Description
technical field [0001] The present invention relates to plant tissue culture, in particular to a kind of pine tissue culture technology. Background technique [0002] Gang pine is also known as: broom branch, chicken pine, sweeping card wood, sand pine, and fragrant firewood. Dwarf shrubs, rarely small trees, up to 1-2 meters high. Much branched, bald. Simple leaves, opposite, erect or extended; leaf blade linear conical, apex pointed, grooved above, raised below, with transparent glandular points. Flowers small, white, solitary in leaf axils, with 2 bracts at base, short stalk; calyx tube bell-shaped, 5-lobed, membranous, persistent; petals 5, round, ovary 3-loculed, with several ovules in each locus . Capsule ca. 1 mm long, dehiscing at upper part. Seeds horned. The flowering period is July to August. The whole herb or root of Pinus pine can be used as medicine, which can be collected throughout the year, dried or used fresh. It grows in hills, mountain streams and...
Claims
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Application Information
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