Culturing technology for upright-ladybell-root in-vitro regeneration tissue

Abstract

The invention discloses a culturing technology for upright-ladybell-root in-vitro regeneration tissue. Upright ladybell roots are campanulaceae plants, and medicinal materials of the upright ladybellroots are dried roots of adenophora tetraphylla or adenophora hunanensis. Digging is carried out in spring and autumn, stem leaves and fibrous roots are removed, soil is cleaned, cork is scraped, anddrying in the sun is carried out. The upright ladybell roots are long conical or long cylindrical, some roots are slightly curved or warped, and a plurality of half-moon-shaped stem marks exist on theperiphery, and are in disc section shapes; surfaces are yellowish white or light brownish yellow, residual brown cork frequently exists at sunken portions, a plurality of deep-sunken transverse striations are arranged at the upper portions and are intermittent annular striations, and longitudinal wrinkles and longitudinal furrows are arranged at the lower portions. The weight is light and porous,bending easily occurs, and fracture surfaces are yellowish white and uneven, and have multiple fractures. The smell is feeble, and the flavor is sweet and light; the roots which are rough and long instrip, dry, rich, yellowish white in color and completely-clean in scarfskin are good. According to the culturing technology, the upright ladybell roots with bud stem sections serve as explants, upright-ladybell-root in-vitro regeneration is achieved through processes such as bud induction, bud enrichment culturing, rooting culturing, seedling hardening and transplanting, and a technical supportis provided for mass breeding, rapid propagation and new-species popularization.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a technology for culture of isolated regenerated tissue of Nansha ginseng. Background technique [0002] Nanshashen is the dried root of Campanulaceae plants, Adenophora verticillum or Adenophora apricot. Excavated in spring and autumn, remove the stems, leaves and fibrous roots, wash the soil, scrape off the cork, and dry in the sun. Properties of medicinal material: The roots are long conical or long cylindrical, some are slightly curved or twisted, and there are many half-moon-shaped stem scars around them, which are disc-shaped. The surface is yellowish-white or light brownish-yellow, and there are often residual tan cork in the depressions. The upper part often has deep horizontal lines, which are intermittent ring lines, and the lower part has longitudinal wrinkles and longitudinal grooves. The body is light and vesicular, easy to break, the cross-section is yellowish white, uneven...

AI Technical Summary

Problems solved by technology

Ginseng seedlings are mainly propagated by seed sowing method. Due to the low germination rate of seeds, it is far from meeting the needs of large-scale production of Ginseng seedlings.

Moreover, there is a difference in medicinal effect between the offspring of seed propagation, which is not conducive to the promotion of good varieties.