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Propagation method of European Chinese rose tissue cultivation

A tissue culture and European moon technology, applied in the field of plant cultivation, can solve the problems of high cost of cutting propagation, low survival rate and long cycle.

Inactive Publication Date: 2019-05-28
天津创世生态景观建设股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ouyue can be propagated by seeds, cuttings and tissue culture techniques, and cuttings are often used for propagation, but the cost of cutting propagation is high, the survival rate is low, the cycle is long, and it is difficult to produce in large quantities, while the tissue culture technology has high reproduction efficiency and short cycle , genetically shape stable and easy to manage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Bud induction culture: take the semi-lignified stem section of Zhenzhou, a classic variety of Ouyue, as an explant, cut off the leaves, cut into 2-3cm long stem sections with 2-3 axillary buds, and use Soak in washing powder solution for 5 minutes, then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 1 hour and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 30 seconds Wash 3 times with sterile water, then sterilize with 0.1% mercuric chloride solution for 8 minutes, rinse 4 times with sterile water, blot dry the water on the surface with sterile filter paper, inoculate into bud induction medium for bud induction, and inoculate at 25 Cultivate in total darkness for 5 days under the condition of ℃, and then place it in light for 10 hours per day, and cultivate for 30 days under the condition of light intensity of 1500 lx, and the bud induction ...

Embodiment 2

[0022] (1) Bud induction culture: take the semi-lignified stem section of Zhenzhou, a classic variety of Ouyue, as an explant, cut off the leaves, cut into 2-3cm long stem sections with 2-3 axillary buds, and use Soak in washing powder solution for 6.5 minutes, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 1.5 hours and place it in an ultra-clean workbench, first disinfect with 75% ethanol After 45 seconds, wash 4 times with sterile water, then sterilize with 0.1% mercuric chloride solution for 9 minutes, rinse 5 times with sterile water, dry the surface water with sterile filter paper, and inoculate into bud induction medium for bud induction. Under the condition of 26° C., it was cultured in total darkness for 6 days, and then placed in light for 11 hours a day, and the light intensity was 2000 lx for 30 days, and the bud induction rate was 80.0%. The bud induction medium is:...

Embodiment 3

[0027](1) Bud induction culture: take the semi-lignified stem section of Zhenzhou, a classic variety of Ouyue, as an explant, cut off the leaves, cut into 2-3cm long stem sections with 2-3 axillary buds, and use Soak in washing powder solution for 8 minutes, then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 60 seconds Wash 5 times with sterile water, then sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse 6 times with sterile water, blot the water on the surface with sterile filter paper, inoculate into bud induction medium for bud induction, and inoculate at 28 Cultivate in total darkness for 7 days under the condition of ℃, and then place it under the condition of 12 hours of light every day, and cultivate for 30 days under the condition of light intensity of 2500 lx, and ...

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PUM

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Abstract

The invention discloses a European Chinese rose tissue cultivation method, and relates to a method of European Chinese rose for rapidly obtaining massive European Chinese rose varieties through planttissue cultivation technology within short time. European Chinese rose stem segments with buds are used as explants, regeneration in vitro of European Chinese roses is realized through the processes of bud induction, bud multiplication cultivation, rooting cultivation, hardening seedling and transplantation, and technical support is provided for massive seed selection, rapid propagation and new species popularization.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation methods, in particular to an Ouyue tissue culture propagation method. Background technique [0002] Rose is one of the top ten famous flowers in my country and belongs to the evergreen or semi-evergreen shrubs of the family Rosaceae. Rose has the advantages of rich color and pattern, long flowering period and strong adaptability, so it is widely used in landscaping. In recent years, due to the improvement of breeding technology and the wide application of transgenic technology, new varieties of roses have emerged continuously, and Ouyue has a good market prospect due to its high ornamental value. These new varieties are rich and colorful in flower and color, a variety of single and complex colors, gradient stripes and spots, and rich and colorful. From the plant type, there are large and small vines, large and small shrubs, and miniatures, which are graceful and charming. Whether thes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙桂梅于杰于丽高萌萌孙伟王洪钰于福满
Owner 天津创世生态景观建设股份有限公司
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