Tissue culture method of sapium sebiferum

Abstract

The invention discloses a tissue culture method of sapium sebiferum. The tissue culture method is characterized in that deciduous trees are 12m high and have latex. Tree barks are gray and have shallow longitudinal cracks. Single leaves alternate and are papery; the leaves have a prismatic shape and a broad rhombic ovum shape; the leaves have long and sharp tips, and basic parts of the leaves havea broad wedge shape and an obtuse shape; full edges and two surfaces of the leaves are green, have no fluff and become red in autumn; the sapium sebiferum blossoms from June to July and has small andgreen-yellow flowers; the fruit period is August to October; capsules are round and spherical and are brown when the capsules are ripe; the capsules are subjected to loculicidal dehiscence to form three pieces, and each piece contains one seed. The seeds are nearly spherical and black and are coated with white wax. The barks can be harvested in the whole year; the barks are peeled off, corks areremoved and the barks are dried in the sun. The sapium sebiferum grows in Eastern China, Southern China and the like. The sapium sebiferum has a bitter taste, is warm in nature and has toxin. The sapium sebiferum has the effects of inducing diuresis, promoting digestion, killing insects and detoxifying. The tissue culture method disclosed by the invention takes stem segments with buds of the sapium sebiferum as explants, and in-vitro regeneration of the sapium sebiferum is realized through processes of bud induction, bud multiplication culture, rooting culture, seedling exercising, transplanting and the like; technical supports are provided for large-scale breeding, rapid reproduction and new variety popularization.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a method for tissue culture of uber. Background technique [0002] Also known as black cypress: wood wax tree, white tallow, black cypress. It is a deciduous tree, up to 12 meters, with latex. Bark gray with shallow longitudinal fissures. Single leaves alternate, papery; prismatic to broad diamond-shaped ovate, apex long and acuminate, base broadly cuneate to blunt, entire, both sides green, glabrous, turning red in autumn; petiole top with 2 glands . Blooms from June to July, the flowers are unisexual, monoecious; the racemes are terminal, the flowers are small, green-yellow, without petals and discs; the fruiting period is from August to October. Capsule ellipsoid, brown when mature, dehiscated into 3 petals, each petal has 1 seed. Seeds subglobose, black, covered with white wax. The skin can be harvested throughout the year, the skin is peeled o...

AI Technical Summary

Problems solved by technology

At present, Ubera seedlings are mainly propagated by seed sowing, but due to the low germination rate of seeds, it is far from being able to meet the needs of large-scale production for Ubera seedlings

Moreover, there are differences in the medicinal components between the offspring of seed propagation, which is not conducive to the popularization of excellent varieties of uber