Tissue culture method of Eucommia ulmoides Oliv.

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of inability to meet large-scale production of Eucommia seedlings, unfavorable promotion of fine Eucommia varieties, and low seed germination rate.

Inactive Publication Date: 2018-06-12
熊小利
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Eucommia seedlings are mainly propagated by seed sowing method, which is far from meeting the needs of large-scale production for Eucommia seedlings due to the low germination rate of the se

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Bud induction culture: use the stem section of Eucommia ulmoides No. 5 with buds as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds, and soak them in washing powder solution for 5 minutes , and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 1 hour and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 5 seconds, and then wash it with sterile water for 3 seconds sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 4 times, and then dried the water droplets on the surface with sterile filter paper, then inoculated into bud induction medium for bud induction. Cultivate in the dark for 3 days, then place it under light for 10 hours every day, and cultivate for 30 days under the condition of light intensity of 1500lx, count its bud induction rate and growth situation, a...

Embodiment 2

[0021] (1) Bud induction culture: take Huazhong No. 5 Eucommia eucommia stem with buds as explants, cut off the leaves, cut into 3.0-5.0 long segments with 2-3 axillary buds and soak them in washing powder solution for 10 minutes , and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface, then rinse it with tap water for 2 hours and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 15 seconds, and then wash it with sterile water for 5 seconds sterilized with 0.1% mercuric chloride solution for 15 minutes, rinsed with sterile water for 4 times, and then dried the water droplets on the surface with sterile filter paper, then inoculated into bud induction medium for bud induction. Cultivate in the dark for 3 days, then place it under light for 10 hours every day, and cultivate for 30 days under the condition of light intensity of 1500lx, count its bud induction rate and growth situation, and ...

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Abstract

The invention discloses a tissue culture method of Eucommia ulmoides Oliv., and relates to a method for obtaining a large number of good Eucommia ulmoides Oliv. variety seedlings quickly within a short time through a plant tissue culture technology of the Eucommia ulmoides Oliv. According to the tissue culture method of the Eucommia ulmoides Oliv., in-vitro regeneration of the Eucommia ulmoides Oliv. is realized through the processes of bud induction, bud propagation culture, rooting culture, seedling hardening, transplanting and the like by taking an Eucommia ulmoides Oliv. stem segment withbuds as an explant, and technical support is provided for large number of breeding, rapid propagation, and new variety promotion.

Description

technical field [0001] The invention relates to a plant tissue culture method in plant biotechnology, in particular to a eucommia tissue culture method. Background technique [0002] Eucommia ulmoides (Eucommia ulmoides Oliv.) is a perennial deciduous tree belonging to the genus Eucommia in the family Eucommia. Eucommia ulmoides is a rare and valuable medicinal plant with various pharmacological effects, and it is also the most ideal Chinese medicinal material for human anti-aging and health care. Its dry bark can be used as medicine. It has the functions of nourishing the kidney, strengthening the waist, strengthening the tendons, strengthening the bones, anti-pregnancy, and lowering blood pressure. It is also known as the top grade of traditional Chinese medicine. In addition, Eucommia is also the raw material for extracting Eucommia gum, which is a natural polymer glue with strong corrosion resistance, excellent insulation performance, good plasticity, and high compressi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 熊小利
Owner 熊小利
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