Application of insulin-like growth factor binding protein 2 in preparation of postoperative prognosis evaluation kit for neuroendocrine carcinoma

A neuroendocrine cancer and growth factor technology, applied in the biological field, can solve problems such as unclear molecular mechanism and lack of specific molecular targets for treatment

Inactive Publication Date: 2018-01-30
FUJIAN NORMAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, the molecular mechanism of gastric neuroendocrine carcinoma is still unclear, and its treatment also lacks specific molecular targets. As insulin-like growth factor-binding protein 2 is a very important tumor oncogene, there is no literature on insulin-like growth factor-binding protein 2. Growth factor binding protein 2 is related to the prognosis or metastasis of gastric neuroendocrine carcinoma

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  • Application of insulin-like growth factor binding protein 2 in preparation of postoperative prognosis evaluation kit for neuroendocrine carcinoma
  • Application of insulin-like growth factor binding protein 2 in preparation of postoperative prognosis evaluation kit for neuroendocrine carcinoma
  • Application of insulin-like growth factor binding protein 2 in preparation of postoperative prognosis evaluation kit for neuroendocrine carcinoma

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Embodiment 1

[0039] A kit for postoperative prognosis assessment of gastric neuroendocrine carcinoma, the kit comprising: human insulin-like growth factor-binding protein 2 for detecting the relative expression of insulin-like growth factor-binding protein 2 in gastric neuroendocrine carcinoma tissue Protein 2 monoclonal antibody or insulin-like growth factor binding protein 2 polyclonal antibody, immunohistochemical reagents. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0040] The human insulin-like growth factor binding protein 2 polyclonal antibody of the present invention is obtained by immunizing rabbits with the insulin-like growth factor binding protein 2 whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0041] Immunohistochemical experiment reagent of ...

Embodiment 2

[0043] The application of insulin-like growth factor binding protein 2 in the preparation of postoperative prognosis evaluation kit for gastric neuroendocrine carcinoma, the application is to use the kit of the present invention to evaluate postoperative prognosis of gastric neuroendocrine carcinoma, the method is as follows:

[0044] (1) Take pathological specimens from patients with gastric neuroendocrine carcinoma, and perform immunohistochemistry using human insulin-like growth factor binding protein 2 monoclonal antibody or human insulin-like growth factor binding protein 2 polyclonal antibody, and immunohistochemical reagents dyeing;

[0045] Among them, the pathological specimens come from the biopsies of patients with gastric neuroendocrine carcinoma or the pathological materials taken during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0046] (a) Paraffin sections of gastric neuroendocri...

Embodiment 3

[0066] Take a gastric neuroendocrine cancer postoperative cell carcinoma tumor sample for paraffin-embedded sections, and use the above-mentioned immunohistochemical method for detection. After calculation, the insulin-like growth factor binding protein 2 immunohistochemical tissue score of the cancer tissue is is 0.752. After postoperative follow-up, it was found that the patient developed recurrence and metastasis of gastric neuroendocrine carcinoma in the 7th month after the operation, and died 10 months after the operation.

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Abstract

The invention provides application of insulin-like growth factor binding protein 2 in preparation of a postoperative prognosis evaluation kit for neuroendocrine carcinoma, the prognosis evaluation kitfor the neuroendocrine carcinoma and a prognosis evaluation method for the neuroendocrine carcinoma. Relative transcript level of the insulin-like growth factor binding protein 2 in neuroendocrine carcinoma tissues is detected according to an immunohistochemical method so as to judge relapse and metastasis risks of the neuroendocrine carcinoma of the neuroendocrine carcinoma patients. The application of the insulin-like growth factor binding protein 2 in preparation of the postoperative prognosis evaluation kit for the neuroendocrine carcinoma has the advantage that the insulin-like growth factor binding protein 2 can be used for preparing protein molecule markers for judging prognosis of the neuroendocrine carcinoma patients, thereby being of great guidance significance in postoperativemonitoring and sequential therapy of the neuroendocrine carcinoma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of insulin-like growth factor binding protein 2 in the preparation of a kit for evaluating the prognosis of gastric neuroendocrine carcinoma after surgery, the kit and method for evaluating the prognosis of gastric neuroendocrine carcinoma. Background technique [0002] Neuroendocrine tumors are a group of heterogeneous tumors originating from various types of neuroendocrine cells and peptidergic neurons. Gastric neuroendocrine neoplasm (GNEN) is a tumor derived from neuroendocrine cells such as enterochromaffin-like cells (ECL) or enterochromaffin (EC) cells, accounting for about all neuroendocrine tumors. 6%, its incidence rate is about 2 / 100 million. In 2010, WHO divided GNEN into three grades: G1, G2 and G3 according to the degree of cell differentiation, mitosis and cell proliferation activity (Ki-67 index), among which G1 and G2 belong to highly diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531G01N33/577
Inventor 林尧黄昌明谢建伟王清水
Owner FUJIAN NORMAL UNIV
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