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A method for preparing an original cell model and a method for simulating the directional foraging behavior of a cell population using the model

A cell population and model simulation technology, applied in teaching models, preparation of microspheres, educational appliances, etc., can solve problems such as inability to simulate the directional foraging behavior of cell populations, and achieve the effect of expanding the types of construction materials

Active Publication Date: 2019-07-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing original cell model cannot simulate the directional foraging behavior of the cell population, and provide a method for preparing the original cell model and a method for simulating the directional foraging behavior of the cell population using the model

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  • A method for preparing an original cell model and a method for simulating the directional foraging behavior of a cell population using the model
  • A method for preparing an original cell model and a method for simulating the directional foraging behavior of a cell population using the model
  • A method for preparing an original cell model and a method for simulating the directional foraging behavior of a cell population using the model

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specific Embodiment approach 1

[0029] Specific embodiment one: what this embodiment records is a kind of preparation method of original cell model, and the specific steps of described method are as follows:

[0030] Step 1: Preparation of PBS buffer solution: NaH 2 PO 4 and Na 2 HPO 4 After mixing, dissolve in ultrapure water to prepare PBS buffer solution; the NaH 2 PO 4 with Na 2 HPO 4 The mass ratio is 1:(1.8~21), the concentration of the PBS buffer solution is 50~100 mmol / L, and the pH value is 7.0~8.0;

[0031]Step 2: preparation of bovine serum albumin and lipase mixed solution: after bovine serum albumin and lipase are mixed, dissolve in the PBS buffer solution obtained in step 1 to obtain bovine serum albumin and lipase mixed solution; The mass ratio of bovine serum albumin and lipase is 1: (0.25~1), and the concentration of the mixed solution of bovine serum albumin and lipase is 2~10 mg / mL;

[0032] Step 3: Preparation of oil phase solution: mixing liquid triglyceride and silicone oil to o...

specific Embodiment approach 2

[0034] Specific embodiment two: the preparation method of a protocell model described in specific embodiment one, in step one, the NaH 2 PO 4 with Na 2 HPO 4 The mass ratio is 1:1.8, the concentration of PBS buffer solution is 50 mmol / L, and the pH value is 7.0.

specific Embodiment approach 3

[0035] Specific embodiment three: the preparation method of a kind of original cell model described in specific embodiment one, in step two, the mass ratio of described bovine serum albumin and lipase is 1: 1, and described bovine serum albumin and lipase The concentration of the enzyme mixed solution was 2 mg / mL.

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Abstract

The invention relates to a preparation method of a protocell model and a method for simulating a directional foraging behavior of a cell population by using the protocell model, belonging to the fields of protocell simulation as well as preparation of biomaterials and biomimetic materials. The methods comprise the following steps: preparing a phosphate buffer solution (PBS); preparing a mixed solution of bovine serum albumin and lipase; preparing an oil phase solution; using a Pickering microemulsion method to prepare an oil-in-water hybrid protein capsule protocell model which takes the bovine serum albumin and the lipase as stabilizers; preparing a food solution; simulating the directional foraging behavior of the population. The preparation method of the protocell model and the method for simulating the directional foraging behavior of the cell population by using the protocell model have the advantages that the types of building materials, i.e., natural protein materials, of the protocell model are expanded, and the protocell model is used for realizing the simulation of the directional foraging behavior of the population, so that the new protocell model is provided for intensively studying the real cell behavior. Furthermore, the scientific problem that the existing protocell model difficultly realizes oriented movement of the population is solved. The methods provided bythe invention provide a new research platform for in-depth study of cell functions and behavioral simulation in the future.

Description

technical field [0001] The invention belongs to the field of protocell simulation, biological material and bionic material preparation, and in particular relates to a method for preparing a protocell model and a method for simulating the directional foraging behavior of a cell population by using the model. Background technique [0002] The original cell model is an artificial cell model that can simulate real cells. The construction of the original cell model is of great significance for the study of real cells and the origin of life. A variety of protocell models based on various materials have been reported, for example, colloidal capsule protocell models based on silica nanoparticles, phospholipid capsule protocell models based on phospholipids, polymerization based on block polymers or hyperbranched polymers Biocapsule protocell model, protein-polymer-based protein capsule protocell model. However, based on the constructed protocell model, the progress of further simul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/06G09B23/00
CPCB01J13/06G09B23/00
Inventor 王磊林幼萍刘小曼黄鑫
Owner HARBIN INST OF TECH