A microfluidic device for biochemical detection
A biochemical detection and microfluidic technology, applied in the field of microfluidics, can solve the problems of affecting the detection effect and insufficient contact of the reaction fluid, and achieve the effects of good reproducibility, thorough reaction, full contact and mixing
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Embodiment 1
[0031] This embodiment specifically illustrates the structure of the microfluidic device of the present invention.
[0032] Such as Figure 1-3 As shown, the microfluidic device of the present invention includes a fluid channel chip and a detection zone 1; the fluid channel chip is used for fluid sampling and mixing, and the fluid channel chip is provided with first micropores 31; The detection liquid enters the detection area 1 through the first microhole 31 for detection.
[0033] Such as Figure 1-3 As shown, the detection area structure in this embodiment includes two detection units.
[0034] image 3 It is a schematic diagram of the structure of the detection area including two detection units. The structure of the detection area includes a cover sheet 11, a first substrate 12, a second substrate 13, and a third substrate 14 from top to bottom; The side of a substrate 12 provided with micro-channels is bonded to a side of the second substrate 13; the second substrate 13 is pro...
Embodiment 2
[0042] The difference between this embodiment and Embodiment 1 is only that the detection area includes at least 3 detection units, and multiple detection units are connected in series or in parallel.
[0043] Such as Figure 4 As shown, Figure 4 (a) is the structure of the detection zone in series, the mixed fluid to be detected enters the first detection unit through the first micropore, then passes through the second...n-1th detection unit in turn, and finally passes through The micropore of the nth detection unit returns to the fluid channel chip and enters the waste liquid pool to complete the detection;
[0044] Figure 4 (b) It is the structure of the detection zone in which the multiple detection units are connected in parallel. The lower surface of the third substrate is also provided with a micro flow channel. After the mixed fluid to be detected passes through the first micro hole, the The flow is split on the micro flow channel and enters the first n-1 detection units ...
Embodiment 3
[0046] This example illustrates the preparation method of the detection zone 1 described in Example 1, and the steps are as follows:
[0047] (1) Using conventional technology, micro-channels are provided on one side of the first substrate; micro-holes are provided on the second substrate and the third substrate;
[0048] (2) Fix biomolecules on a large-area membrane, and then cut the large-area membrane into membrane units with biomolecules immobilized as a biomolecule coating film;
[0049] (3) The cover sheet, the first substrate, the second substrate and the third substrate are sequentially bonded from bottom to top, and the biomolecule coating film is fixed between the second substrate and the third substrate in an embedded form, Then assembled and fixed to the control layer.
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