In-vitro culture and rapid propagation method of lithops
A kind of technology of in vitro culture, Lithops
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[0033] The present embodiment provides a method for establishing a complete tissue culture and rapid propagation system of Lithops spp. of the family Apricotaceae, including the following steps:
[0034] Step 1, Lithops explant treatment:
[0035] The mature leaves of the succulent Lithops are selected as explants, and the mature female plants with a diameter of about 2 to 4 cm are soaked in detergent for 15 minutes and washed with running water for more than 30 minutes. In the ultra-clean workbench, 75% ethanol and 2% NaClO solution were used for disinfection treatment. The callus of the explants treated for 5s to 8min expanded and differentiated, and the sterility rate reached 41.2%. The rate is as high as 45.6%.
[0036] Table 1 The effect of different disinfection concentrations on the callus of Lithops
[0037]
[0038] Note: Different lowercase letters indicate that the difference is not significant at the 0.05 level.
[0039] Step 2. Start training:
[0040] Inoc...
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