Application of 6-azauracil to preparation of fungicide for preventing and treating plant diseases caused by phytopathogen
A plant pathogenic bacteria, nitrogen uracil technology, applied in the direction of chemicals, fungicides, plant growth regulators, etc. for biological control, can solve the problems of no reports, etc., to ensure high quality, large environmental pollution, poor drug resistance Effect
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[0041] 1. Experimental materials
[0042] The 6-azuracil agent was a finished product purchased from Sigma. According to the specific requirements of the experiment, 6-nitrouracil was weighed and dissolved in dimethyl sulfoxide to prepare a 100 mM / L mother solution, which was sterilized by filtration and stored at 4°C until use.
[0043] The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato medium (PDA for short).
[0044] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.
[0045] Preparation method: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter it into a beaker with eight layers of gauze, add 15-20g of agar, add 20g of glucose, stir evenly, fully dissolve and then cool down to make up water to 1000ml, after subpackaging, sterilize at 121°C f...
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