Cantharidin extraction method and application thereof
An extraction method and technology for cantharidin, which are applied in pharmaceutical formulations, cosmetic preparations, cosmetics, etc., can solve the problems of harming the human body or the environment, long extraction period, low extraction rate, etc., achieve good impurity removal effect, and promote solvent penetration. , the effect of high solubility
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Embodiment 1
[0026] A method for extracting cantharidin, comprising the following steps:
[0027] (1) collect adult mylabris, wash, dry and pulverize and cross a 35-mesh sieve to get the cantharis powder;
[0028] (2) Add distilled water to the mylabris powder in step (1), the mass volume ratio (g / mL) of mylabris powder to distilled water is 1:5, then add 0.2% papain, shake well and heat up to 35°C, stir and shake , enzymatic hydrolysis for 9 hours, filtered, and collected filter residue;
[0029] (3) Add distilled water to the filter residue of step (2), the mass volume ratio (g / mL) of filter residue to distilled water is 1:6, then add 0.35% lipase, enzymolyze at 40°C for 9h, filter and collect filter residue;
[0030] (4) Add ethyl acetate to the filter residue of step (3), the mass volume ratio (g / mL) of the filter residue to ethyl acetate is 1:13, at a temperature of 45°C, a power of 250W, and a frequency of 50kHz After ultrasonic extraction for 25 minutes, filter through a micropor...
Embodiment 2
[0033] A method for extracting cantharidin, comprising the following steps:
[0034] (1) collect the adult mylabris, wash, dry and pulverize through a 30-mesh sieve to get the cantharis powder;
[0035] (2) add distilled water in the mylabris powder of step (1), the mass volume ratio (g / mL) of mylabris powder and distilled water is 1:, add the papain of 0.1% again, shake up to 30 ℃, stir and shake, Enzymolysis for 10 hours, filtering, and collecting the filter residue;
[0036] (3) Add distilled water to the filter residue of step (2), the mass volume ratio (g / mL) of filter residue to distilled water is 1:1, then add 0.2% lipase, enzymolyze at 35°C for 10h, filter and collect filter residue;
[0037] (4) Add ethyl acetate to the filter residue of step (3), the mass volume ratio (g / mL) of the filter residue to ethyl acetate is 1:10, at a temperature of 40°C, a power of 200W, and a frequency of 40kHz After ultrasonic extraction for 30 minutes, filter through a microporous mem...
Embodiment 3
[0040] A method for extracting cantharidin, comprising the following steps:
[0041] (1) collect adult mylabris, wash, dry and pulverize and cross a 40-mesh sieve to get the cantharis powder;
[0042] (2) Add distilled water to the mylabris powder in step (1), the mass volume ratio (g / mL) of mylabris powder to distilled water is 1:10, then add 0.3% papain, shake well and heat up to 40°C, stir and shake , enzymolysis for 8h, filtering, and collecting the filter residue;
[0043] (3) Add distilled water to the filter residue of step (2), the mass volume ratio (g / mL) of filter residue to distilled water is 1:10, then add 0.5% lipase, enzymolyze at 45°C for 8h, filter and collect filter residue;
[0044] (4) Add ethyl acetate to the filter residue of step (3), the mass volume ratio (g / mL) of the filter residue to ethyl acetate is 1:15, at a temperature of 50°C, a power of 300W, and a frequency of 60kHz After ultrasonic extraction for 20 minutes, filter through a microporous mem...
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