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Stabilizer for detecting alpha-hydroxybutyrate dehydrogenase detection kit and preparation method thereof

A technology of hydroxybutyrate dehydrogenase and stabilizer, which is used in the measurement of color/spectral properties, chemical analysis by catalysis, instruments, etc., can solve the problems of poor stability and low accuracy, and ensure the accuracy , low cost, simple preparation effect

Active Publication Date: 2018-05-04
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current kits on the market have problems of poor stability and low accuracy

Method used

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  • Stabilizer for detecting alpha-hydroxybutyrate dehydrogenase detection kit and preparation method thereof
  • Stabilizer for detecting alpha-hydroxybutyrate dehydrogenase detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A stabilizer for α-hydroxybutyrate dehydrogenase kit, including the following components: 2.00g / L edetate disodium, 10.00g / L sucrose, 10.00g / L Triton X-100, 30.00g / LCCD, 10.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0024] Weigh each component according to any one of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

[0025] Example 1 was applied to the α-hydroxybutyrate dehydrogenase assay kit, and evaluated from two aspects of long-term stability, validity period and usage amount.

[0026] (1) Stability, accuracy and pr...

Embodiment 2

[0051] A stabilizer for α-hydroxybutyrate dehydrogenase kit, including the following components: 2.50g / L edetate disodium, 15.00g / L sucrose, 12.00g / L Triton X-100, 40.00g / LCCD, 12.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0052] Weigh each component according to any one of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

Embodiment 3

[0054] A stabilizer for α-hydroxybutyrate dehydrogenase kit, including the following components: 3.00g / L edetate disodium, 20.00g / L sucrose, 10.00g / L Triton X-100, 35.00g / LCCD, 10.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0055] Weigh each component according to any one of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

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Abstract

The invention discloses a stabilizer for a detecting alpha-hydroxybutyrate dehydrogenase detection kit and a preparation method thereof, belonging to the in vitro diagnosis field. The stabilizer contains the following components in percentage by weight: 0.1%-0.5% of disodium edetate, 1%-3% of saccharose, 1%-2% of Triton X-100, 3%-5% of CCD, 1%-2% of glycerin, 0.1%-0.2% of liquid biological preservative Proclin300 and the balance of water. The stabilizer is applied to the detecting alpha-hydroxybutyrate dehydrogenase detection kit, so that the accuracy of a detection result of the kit is guaranteed, the detecting alpha-hydroxybutyrate dehydrogenase detection kit is relatively stable, and the preservation time of the kit is effectively prolonged. Besides, the stabilizer is low in cost, easyto prepare and worthy of being further widely popularized.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and in particular relates to a stabilizer used in an α-hydroxybutyrate dehydrogenase assay kit and a preparation method thereof. Background technique [0002] α-Hydroxybutyrate dehydrogenase (α-HBDH) is an enzyme in the myocardial enzyme spectrum, which is ubiquitous in mammals and mainly distributed in tissues or organs such as myocardial red blood cells, white blood cells and kidneys. α-Hydroxybutyrate dehydrogenase (α-HBDH) is not an independent specific enzyme, but a general term for lactate dehydrogenase isozymes LDH1 and LDH2 containing H subunits. Determination of α-hydroxybutyrate dehydrogenase actually reflects the activity of lactate dehydrogenase isoenzymes LDH1 and LDH2, which is of great significance for the diagnosis of myocardial disease and liver disease. Increased α-HBDH activity is common in acute myocardial infarction, skeletal muscle injury, acute hepatitis, leukemia and mal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N31/10G01N35/00
CPCG01N21/31G01N31/10G01N35/00
Inventor 黄小全崔海林曾晓君黄清媚
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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