Quality detection method of traditional Chinese medicine compound with efficiency of treating acute and chronic pyelonephritis and prostatic hyperplasia

A quality detection method and technology for benign prostatic hyperplasia, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of single detection method, inability to comprehensively and objectively detect quality, and complex composition of traditional Chinese medicine preparations, and achieve the effect of optimizing mass spectrometry conditions.

Active Publication Date: 2018-05-04
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The composition of the traditional Chinese medicine preparation of the present invention is complex, relying on a single detection method, it is impossible to detect its quality comprehensively and objectively

Method used

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  • Quality detection method of traditional Chinese medicine compound with efficiency of treating acute and chronic pyelonephritis and prostatic hyperplasia
  • Quality detection method of traditional Chinese medicine compound with efficiency of treating acute and chronic pyelonephritis and prostatic hyperplasia
  • Quality detection method of traditional Chinese medicine compound with efficiency of treating acute and chronic pyelonephritis and prostatic hyperplasia

Examples

Experimental program
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Effect test

Embodiment 1

[0063] 1, a kind of quality detection method of the Chinese medicine compound prescription that has treatment acute and chronic pyelonephritis and prostatic hyperplasia effect, it is characterized in that, comprises the following steps:

[0064] (1) Preparation of reference substance solution

[0065] Accurately weigh the reference substances of timosaponin BII, timosaponin AⅢ, cortex cortex, jatrorrhizine, berberine hydrochloride, palmatine hydrochloride, mangiferin and neomangiferin, place them in a 10mL volumetric flask, and use The volume concentration of 70% acetonitrile was fixed to the mark, and the individual controls with mass concentrations of 1006 μg / mL, 999 μg / mL, 1288 μg / mL, 1251 μg / mL, 4004 μg / mL, 1775 μg / mL, 1258 μg / mL and 1114 μg / mL were prepared Product stock solution;

[0066] Precisely draw appropriate amount of timosaponin BII reference substance stock solution, timosaponin AⅢ reference substance stock solution, cortex base stock solution, jatrorrhizine st...

Embodiment 2

[0100] Example 2 Sample Determination

[0101] The preparation method of Zishen Tongguan Capsules is as follows: take the three herbs of Phellodendron Phellodendri, Zhimu and Cinnamon;

[0102] Take 50g of cinnamon and crush it into fine powder, set aside;

[0103] Take 500g of Phellodendron cortex, crush it into coarse powder, add 65% ethanol to soak for 24 hours, then percolate, control the flow rate to 2ml / min, collect the percolate until the color is light, and set aside;

[0104] Take 500g Anemarrhena, add 2500ml65% ethanol to impregnate for 24 hours, filter the impregnation solution, add 65% ethanol to the dregs and reflux extraction twice, add 2500ml65% ethanol each time, heat and reflux for 2 hours, filter, combine the filtrate, and the filtrate and Phellodendron percolate was combined, ethanol was recovered under reduced pressure and concentrated to a thick paste. Take the thick paste, add an appropriate amount of dextrin (the amount of dextrin in the dry extract mu...

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Abstract

The invention discloses a quality detection method of a traditional Chinese medicine compound with the efficiency of treating acute and chronic pyelonephritis and prostatic hyperplasia. The method comprises the following steps of (1) reference substance solution preparation; (2) internal standard solution preparation; (3) test sample solution preparation; (4) linear relationship investigation; (5)test sample solution detection. According to the structure and property characteristics of eight compounds such as saponin and alkaloid in the traditional Chinese medicine compound, through a large number of experiments, analysis conditions such as optimal chromatographic column and mobile phase composition, elution programs, flow speed, chromatographic columns and mass spectrum are screened out.Multiple experiments prove that the method can simultaneously detect eight compounds such as the saponin and the alkaloid. The method has the advantages that the detection sensitivity is high; the stability is good; the detection evaluation can be objectively, comprehensively and accurately performed; important significance is realized for controlling the quality and ensuring the clinical safetyand effectiveness.

Description

technical field [0001] The invention relates to a quality control method of a traditional Chinese medicine preparation, in particular to a quality detection method of a traditional Chinese medicine compound with efficacy in treating acute and chronic pyelonephritis and prostatic hyperplasia. Background technique [0002] The Zishen Tongguan Capsule prepared from cinnamon, Cortex Phellodendri and Anemarrhena is an in-hospital preparation of Jiangsu Provincial Hospital of Traditional Chinese Medicine. Zishen Tongguan Capsule has the functions of nourishing the kidney and clearing heat, clearing Qi and clearing the customs in removing dampness and heat in the lower energies, and assisting in gasification of the bladder. The TCM dialectical theory of the present invention is: Anemarrhena anemarrhena in the prescription is bitter and cold, the upper part clears lung heat and reduces fire, the lower part moisturizes kidney dryness and nourishes yin; Cortex Phellodendri is bitter a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘顺周琴妹倪文澎邹恺平陈晓斌管敏孙蕾
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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