Deafness gene detection kit and non-invasive prenatal deafness gene detection method

Abstract

The invention discloses a deafness gene detection kit. The deafness gene detection kit is suitable for detection of a GJB3 gene locus, a GJB2 gene locus, a mitochondrial DNA locus and a SLC26A4 gene locus. The detection kit comprises primers of GF1 to GF9 and GR1 to GR9. The probes comprise sequence probes P1 to P18. Each sequence probe comprises a wild-type probe and a mutant probe. The deafnessgene detection kit realizes non-invasive prenatal detection for pregnant women and early finds deaf children.

Description

technical field [0001] The invention belongs to the field of life science and biotechnology, and specifically relates to a deafness gene detection kit and a prenatal noninvasive deafness gene detection method, wherein the prenatal noninvasive deafness gene detection method accurately judges the fetus by detecting free DNA in the peripheral blood of pregnant women Genotype, early detection of deaf children. Background technique [0002] Congenital deafness is one of the most common birth defects in newborns, with an incidence rate of 0.1% to 0.3%. According to statistics, 60% of patients' deafness is related to genetic factors. According to whether the patient is accompanied by other organ diseases, it can be divided into syndromic deafness and non-syndromic deafness. Non-syndromic deafness can be divided into autosomal dominant inheritance, autosomal recessive inheritance, X-linked and Mitochondrial maternal inheritance. [0003] Among non-synthetic deafness patients in di...

AI Technical Summary

Problems solved by technology

[0005] However, the pre-pregnancy detection of the existing technology can only provide a theoretical probability for whether the newborn is carrying or causing the disease. Most genetic testing during pregnancy cannot determine the proportion of the fetus carrying the mutant gene when the mother carries the deaf gene. diagnosis

In other words, even if families who are planning to conceive do systematic pre-pregnancy testing before pregnancy, the accuracy of their pre-pregnancy testing results cannot be guaranteed

[0006] However, for the prenatal detection of the existing technology, there is a certain detection risk.

The prenatal detection in the prior art mainly detects whether the fetus carries a disease-causing gene through methods such as fetal chorionic villi or amniocentesis. This kind of prenatal detection has certain risks for both the fetus and the pregnant woman. also limits the way of invasive prenatal testing

Images