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Application of miR-184 in early warning of spontaneous abortion

A technology of 1. mir-184, repeated spontaneous abortion, applied in the field of medicine, can solve problems such as unsatisfactory curative effect

Inactive Publication Date: 2018-05-18
SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The etiology mainly includes chromosomal abnormalities, maternal reproductive tract abnormalities, maternal endocrine abnormalities, immune function abnormalities, reproductive tract infections, cervical insufficiency, and thrombosis tendency, etc. However, 50% of patients with recurrent spontaneous abortions cannot be diagnosed using current clinical diagnostic methods. If the cause of repeated miscarriages is discovered and the curative effect is not satisfactory, it is called unexplained recurrent spontaneous abortion, which is a clinically difficult infertility disease.

Method used

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  • Application of miR-184 in early warning of spontaneous abortion
  • Application of miR-184 in early warning of spontaneous abortion
  • Application of miR-184 in early warning of spontaneous abortion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Analysis of the expression of miR-184 in the villi and decidua tissues of normal pregnant women and patients with recurrent spontaneous abortion

[0029] The villi and decidua tissues of normal pregnant women and patients with recurrent spontaneous abortion were collected for microRNA chip analysis (Boao Biological Co., Ltd.), and the SAM program package was used to screen according to the criteria of q-value≤5% and Fold Change≥5 or ≤0.2 The results showed that miR-125b-2, miR-187, and miR-184 were specifically highly expressed in villi tissues of patients with recurrent spontaneous abortion, and miR-4672, miR-3175, and miR-520f were specifically lowly expressed ( figure 1 A), while miR-520h, miR-517c, miR-519a-1 / / miR-519a-2, miR-522, and miR-184 were specifically highly expressed in the decidua tissue of patients with recurrent spontaneous abortion ( figure 1 B). It can be seen that miR-184 is specifically highly expressed in the villi and decidual tissues o...

Embodiment 2

[0030] Example 2 Analysis of the expression of miR-184 in the peripheral blood of normal pregnant women and patients with recurrent spontaneous abortion

[0031] At the same time, the peripheral venous blood of normal pregnant women and patients with recurrent spontaneous abortion was collected to detect the expression level of miR-184 (Shanghai Qiming Information Technology Co., Ltd.), and it was found that the expression of miR-184 in the peripheral blood of patients with recurrent spontaneous abortion also increased ( figure 2 ).

Embodiment 3

[0032] Example 3 miR-184 overexpression can promote trophoblast cell apoptosis

[0033] The negative control lentivirus with GFP fluorescent protein and the miR-184 overexpression lentivirus (purchased from Suzhou Gemma Gene Co., Ltd.) were used to infect the human trophoblast cell line HTR8 cells, and the miR-184 overexpressed HTR8 cells were constructed ( image 3 A-B), cell apoptosis was detected by AnnexinV staining (AnnexinV apoptosis kit was purchased from Biolgend Company), and it was found that the overexpression of miR-184 could promote the apoptosis of HTR8 cells ( image 3 C).

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Abstract

The invention belongs to the technical field of medicines, relates to application of miR-184 in early warning of spontaneous abortion and particularly relates to a method for establishing a spontaneous abortion model by intravenous injection of miR-184-3p agomir and detection of miR-184 in peripheral blood of pregnant women in early pregnancy for evaluating the risk of spontaneous abortion. Shownby in vitro experiments, high expression of miR-184 in a maternal-fetal interface in early pregnancy can induce occurrence of spontaneous abortion by a related molecular mechanism, and the expressionof miR-184 in the peripheral blood of patients with recurrent spontaneous abortion also increases; and shown by in vivo animal experiments, after intravenous injection of miR-184-3p agomir induces overexpression of miR-184 in vivo of a mouse, the absorption rate of a mouse embryo increases, thereby establishing a spontaneous abortion animal model. In addition, compared with normal pregnant women,the expression of miR184 in the peripheral blood of patients with recurrent spontaneous abortion increases, and a method for evaluating the risk of spontaneous abortion in early pregnancy is provided.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of miR-184 in early warning of spontaneous abortion. Background technique [0002] Successful pregnancy is an extremely complex physiological process. As the frontier of direct contact between the embryo and the mother, the normal biological function of the mother-fetal interface is the key to maintaining a successful pregnancy. In recent years, the immune regulation mechanism of the mother-fetal interface and how to maintain a successful pregnancy It has always been a research hotspot in reproductive immunology. A series of changes occurred at the maternal-fetal interface during early pregnancy, mainly including decidualization of the endometrium, trophoblast development, placenta formation, and maternal-fetal interaction. The matrix is ​​mediated, thus forming a complex network of interactions that regulate the progression of pregnancy. The embryon...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A01K67/027
CPCA01K67/0275A01K2207/05A01K2227/105A01K2267/03C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 金莉萍张媛周济张进平李明清周文洁
Owner SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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