Cleaning light-resisting airing box for immunohistochemical stain experiment

An immunohistochemical and experimental technology, applied in the field of cleaning and drying boxes that are protected from light, can solve the problems of inability to guarantee the effect of avoiding light after incubation with secondary antibodies, different degrees of drying, humidity, etc., so as to reduce the probability of fluorescence quenching. , Improve the degree of cleaning, reduce the effect of dry cracking

Inactive Publication Date: 2018-06-01
FUDAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At this stage, after incubation of the secondary antibody, the tissue section is placed under a light-proof iron box or platinum paper, especially this operation process is more inconvenient after sealing the slide and before drying, because the drying operation will not only cause the fluorescence of the secondary antibody Quenching will also lead to different drying degrees of the films (some films are dry an

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  • Cleaning light-resisting airing box for immunohistochemical stain experiment
  • Cleaning light-resisting airing box for immunohistochemical stain experiment
  • Cleaning light-resisting airing box for immunohistochemical stain experiment

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[0029] The present invention will be further described below through specific embodiments.

[0030] The washing and light-proof drying box designed by the present invention has a housing size of: length 30 cm * width 22 cm * height 4 cm. It is divided into the control panel part, the incubation and cleaning part, the drying part, the moisturizing layer and the electric board layer.

[0031] (1) The box shell can be made of plastic. The box body is coated with light-proof material to form a light-proof layer.

[0032] (2) Control panel: length 30cm*width 2cm, with switch 1, temperature and humidity adjustment button 3. In addition, there is a display screen 2 for displaying time, temperature and humidity. The cover plate 5 plays a role of sealing and avoiding light. In order to facilitate the movement, a handle 4 is provided on the cover plate 5.

[0033] (3) Incubation and cleaning part: The incubation tank 11 has a diameter of 1.5 cm, a depth of 3 cm, and an arc-shaped bottom. Th...

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Abstract

The invention belongs to the technical field of bioexperiments, and particularly relates to a cleaning light-resisting airing box for the immunohistochemical stain experiment. The cleaning light-resisting airing box comprises an outer shell, a control panel, a hatching cleaning device, an airing device, a moisturizing layer and an electric plate layer, wherein the outer shell is a rectangular boxbody, the hatching cleaning device, the airing device, the moisturizing layer and the electric plate layer are arranged in the box body of the outer shell, the control panel is arranged on one side ofthe upper surface of the box body of the outer shell, a cover plate used for sealing and covering the box body is arranged adjacent to the control panel on the upper surface of the box body of the outer shell; an on-off key and a temperature and humidity regulating button are arranged on the control panel; a display screen is further arranged to display time, temperature and humidity. The cleaning light-resisting airing box integrates the three functions of resisting light, cleaning and airing, the probability of fluorescence quenching is reduced, the dust pollution is reduced, a sheet is prevented from cracking, the degree of cleanliness of the sheet is improved, and the experiment effect of the immunofluorescence is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of biological experiments, and in particular relates to a cleaning and light-proof drying box for immunohistochemical staining experiments. Background technique [0002] Immunohistochemical staining is the most common experimental technique. Among them, the whole process after incubation of the secondary antibody needs to be protected from light. At this stage, after incubation of the secondary antibody, the tissue section is placed under a light-proof iron box or platinum paper, especially this operation process is more inconvenient after sealing the slide and before drying, because the drying operation will not only cause the fluorescence of the secondary antibody Quenching will also lead to different drying degrees of the films (some films are dry and cracked, and some films are still wet), which will affect the effects of sealing and staining of the films. [0003] Existing experimental materials canno...

Claims

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Application Information

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IPC IPC(8): G01N1/31G01N33/533G01N21/64F26B9/06
CPCF26B9/06G01N1/31G01N21/6428G01N33/533G01N2021/6432
Inventor 刘婷婷
Owner FUDAN UNIV
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