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Preparation method and application of flavobacterium and flavobacterium secreta

A technology of flavonoids and secretions, applied in the field of microorganisms, can solve the problems of insufficient control of red tide and low number of algae-lytic bacteria.

Active Publication Date: 2018-06-08
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides an application and preparation method of Flavobacterium and secretions, aiming to solve the problem that the number of algae-dissolving bacteria is too small to control red tide

Method used

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  • Preparation method and application of flavobacterium and flavobacterium secreta
  • Preparation method and application of flavobacterium and flavobacterium secreta

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preparation example Construction

[0021] The invention provides a method for preparing the secretion of Flavobacterium, the method comprising:

[0022] Step 1. Cultivate the Flavobacterium in the medium for at least 24 hours, centrifuge the medium, take the supernatant, filter, and sterilize the filtered supernatant at 110-120°C;

[0023] Preferably, the culture time is 24-72 hours. The sterilization temperature is 121°C. The formula of the medium is: 5-6g / L peptone, 1-1.5g / L yeast extract, 0.1-0.2g / L ferric phosphate and 10-12g / L agar powder, wherein the solvent is old sea water. The pH value of the medium is 7.6-7.8.

[0024] Step 2: removing polysaccharides in the sterilized supernatant, desalting, separating, and evaporating to dryness to obtain secretions.

[0025] Further, step two specifically includes:

[0026] Inoculate the Flavobacterium species into the sterile liquid medium, and culture it for at least 48 hours at 23-26° C. with a shaker speed of 150-200 rpm.

[0027] Collect the fermentation ...

Embodiment

[0032] 1. Isolation of Flavobacterium

[0033] 1) Akashiwo sanguinea (Akashiwo sanguinea) was cultured for 30 days under continuous light at 22°C with a light intensity of 3000 Lx using the culture medium to obtain algae liquid with a cell concentration of 2×104cells / mL.

[0034] Among them, the composition of the medium is 5 mg of NaH 2 PO 4 .H 2 O, 75mg NaNO3 , 0.22mg ZnSO 4 .7H 2 O, 0.01 mg CoCl 2 .6H 2 O, 0.18 mg MnCl 2 .4H 2 O, 0.006mg NaMoO 4 .2H 2 O, 4.36 mg Na 2 EDTA.2H 2 O, 3.15 mg FeCl 2 .6H 2 O, 0.01 mg CuSO 4 .5H 2 O, 0.1mg of vitamin B 1 , 0.5ug of vitamin B 12 , 0.1ug of Biotin (H) was dissolved in aged sea water to a volume of 1L. Hakata algae was provided by the Marine Algae Species Laboratory of Jinan University.

[0035] 2) Water samples were collected from the sea area of ​​Dongshan, Shenzhen, where the red tide broke out, using high-temperature sterilized glass bottles with blue caps, and transferred to a refrigerator at 4°C within 2 hour...

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Abstract

The invention provides a preparation method and application of flavobacterium and flavobacterium secreta and relates to the technical field of microorganisms. The flavobacterium has a preservation number of M2017262. The flavobacterium secreta have alga-lysing activity, can inhibit and kill algae in red tide and can well treat red tide.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method and application of Flavobacterium and Flavobacterium secretion. Background technique [0002] Red tide is a serious marine ecological environment disaster on a global scale, which has attracted widespread attention from all walks of life. Due to the intensification of coastal pollution in recent years, eutrophication has occurred in offshore waters, resulting in frequent outbreaks of red tide. When red tide algae multiply, the photosynthesis of algae The effect consumes a large amount of CO 2 , leading to changes in the pH of the water body, affecting the normal life activities of marine organisms. At the same time, because the red tide organisms cover the upper layer of the water, it is difficult for sunlight to reach the depth of the water body, reducing the transparency of the water body, causing the aquatic plants, coral reefs and aquat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C02F3/34C12R1/20
CPCC02F3/34C02F2303/04C12P1/04C12N1/205C12R2001/20
Inventor 陈辉蓉童晶胡章立黎双飞王立岩
Owner SHENZHEN UNIV
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