Method for determining content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography)

A technology of RP-HPLC and Maoliangzhen, which is applied in the field of chemical detection, can solve the problems of content and authenticity, and the quality control indicators of Liangmianzhen are not perfect, and achieve good precision and repeatability, small damage and good separation effect Good results

Inactive Publication Date: 2018-06-12
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the quality control indicators of LMZ have not been perfected, and the quality of LMZ herbs used in the preparation of LMZ toothpaste may vary. However, the formulation process of LMZ toothpaste is strictly kept secret, and there is no relevant report on its quality standard. Therefore, the selection of the raw material drug LMZ will also affect the content and authenticity of the effective components of LMZ toothpaste.

Method used

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  • Method for determining content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography)
  • Method for determining content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography)
  • Method for determining content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography)

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1 Instruments and reagents

[0035] 1.1 Instrument

[0036] Agilent bio-inert quaternary liquid chromatograph (instrument model: Agilent 1260; manufacturer: Agilenttechnology);

[0037] Diode Array Detector (DAD);

[0038] Electronic analytical balance (instrument model: CPA225D; manufacturer: Sartorius Scientific Instruments (Beijing) Co., Ltd.);

[0039] Ultrasonic cleaner (instrument model: Elmasonic P180H; manufacturer: Elma Schmidbauer GmbH, Germany);

[0040] Ultrapure water meter (instrument model: ELGA PURELAB ClassicUVF; manufacturer: Veolia Group);

[0041] Heating magnetic stirrer (instrument model: C-MAG HS 7; manufacturer: IKA, Germany);

[0042] High-performance general-purpose desktop centrifuge (instrument model: AllegraX-15R; manufacturer: Beckman Company of the United States);

[0043] Desktop refrigerated centrifuge (instrument model: 3K15; manufacturer: Sigma, Germany).

[0044] 1.2 Reagent

[0045] Maoliangmianzhen reference substance (provided...

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PUM

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Abstract

The invention discloses a method for determining the content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography). The method has the advantages of simplicity and convenience in operation, rapidness, accuracy, less interference, good precision and repeatability and the like. The invention belongs to the field of chemical testing. The method adopts ethanol to extract toddalolactone in shinyleaf pricklyash root toothpaste, and collects ethanol extract; the RP-HPLC method is adopted to determine the content of toddalolactone in the ethanol extract. The method can provide an experimental basis for the determination of the content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a RP-HPLC method for measuring the content of lianmianzhen in liangmianzhen traditional Chinese medicine toothpaste. Background technique [0002] Liangmianzhen is the dried root of Zanthoxylum nitidum (Roxb.) DC., a Rutaceae plant. It can promote blood circulation and remove blood stasis, promote qi and relieve pain, dispel wind and collaterals, detoxify and reduce swelling. Do not overdose [1] , the variant of Maoliangmianzhen [2] , LMZ medicinal materials are different from Mao LMZ in terms of chemical composition and content. Maoliangzhensu, also known as Feilongzhang blood lactone, is a unique component of Maoliangzhen and Feilongzhang Xuegen; Lianmianzhen chloride is one of the main active ingredients of Liangmianzhen; it exists in medicinal materials Liangmianzhen, Maoliangmianzhen and Feilongzhang in the blood, but the content is different among the three [3-5] . "Ch...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 陈学松钟水桥梁峰
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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