Bacillus licheniformis, inoculant containing the same and application thereof, vomitoxin degradation method and kit

Abstract

Relating to the field of microorganisms, the invention discloses a Bacillus licheniformis, an inoculant containing the same and application thereof, a vomitoxin degradation method and a kit. Specifically, the invention provides a Bacillus licheniformis strain with a preservation number of CGMCC NO.13314. The Bacillus licheniformis provided by the invention can efficiently and quickly degrade vomitoxin in grain and oil and / or feed, especially, when the vomitoxin content of grain and oil and / or feed is up to 50ppm or above, the Bacillus licheniformis also can efficiently and quickly degrade vomitoxin, and has no influence to the palatability of rain and oil and / or feed. At the same time, degradation of vomitoxin with the Bacillus licheniformis has no generation of toxic product, and is safeand environment-friendly. Therefore, the Bacillus licheniformis has good application prospects.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus licheniformis, a bacterium containing the bacterium and its application, a method and a kit for degrading vomitoxin. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (deoxynivalenol, DON), the chemical name is 3α, 7α, 15-trihydroxy Fusarium-9-en-8-one, because it can cause pigs Named after vomiting, it is mainly trichothecene toxins produced by Fusarium graminearum and Fusarium culmorum infecting wheat, barley, oats, corn and other grains. Worldwide, vomitoxin is one of the main contaminating mycotoxins in grain, feed, and food, seriously affecting the health of humans and livestock. After humans and animals ingest food contaminated by vomitoxin, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, and unresponsiveness. In severe cases, it will damage the hematopoietic system and...

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Problems solved by technology

Patent application CN103243047A discloses a strain of Bacillus subtilis that efficiently degrades deoxynivalenol and its application. 900 μL of Bacillus subtilis ANSB471 fermentation broth is reacted with 100 μL of deoxynivalenol (100 μg / ml), and the degradation rate of deoxynivalenol for 2 hours is 25 %, the degradation rate of vomitoxin in 24 hours of reaction is 56%, and the degradation rate of vomitoxin in 48 hours of reaction is 80%, and the degradation rate still needs to be improved

[0005] In addition, most of the existing biological methods for degrading vomitoxin are carried out under mild conditions (such as a temperature of 25-37°C and not exceeding 40°C, and a pH of about 7), however, under higher temperature loads (such as During transport in containers or during feed pelleting) or harsh acidic and alkaline conditions, there is no better solution, which limits the scope of application of biological methods in the degradation of vomitoxin

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