Application of Cordyceps bacterial strain fermentation products to feed additive for improving breeding performance of cocks
A feed additive and reproductive performance technology, which is applied in the field of livestock and poultry breeding to achieve the effects of increasing the amount of semen, reducing the deformity rate and realizing the reproductive performance of roosters.
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Embodiment 1
[0042] 1. Fermentation liquid
[0043] (1) Cordyceps gunis strain: Acremonium terricola MKL18 (can be purchased from Hefei Miikeluo Biological Engineering Co., Ltd.);
[0044] (2) Seed cultivation:
[0045] The medium for seed culture is: 600g white granulated sugar, 300g silkworm chrysalis powder, 150g peptone, 200g soybean powder, 100g yeast powder, 10g MgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0046] The conditions of seed fermentation are as follows: temperature is 25°C, ventilation rate is 1.00L / (L·min), inoculum size is 5% (mass fraction), liquid volume in 30L airlift fermentation tank is 67%, and fermentation time is 24h.
[0047] (3) Expansion of training:
[0048] The culture medium for expanded cultivation is: 6000g white granulated sugar, 3000g silkworm chrysalis powder, 1500g peptone, 2000g soybean powder, 1000g yeast powder, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, na...
Embodiment 2
[0062] In this embodiment, Acremonium terricola (Acremonium terricola) MKL18 is used as the fermentation object for fermentation to obtain fermentation broth, fermentation powder and solid culture; except that the medium used for liquid fermentation is different from that of Example 1, the remaining contents are the same as those in Example 1. Example 1 is the same.
[0063] The medium for seed culture is: 600g white granulated sugar, 550g yeast powder, 200g soybean powder, 10g MgSO 4 , 10gKH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0064] The culture medium for expansion is: 6000g white granulated sugar, 5500g yeast powder, 2000g soybean powder, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, natural pH; sterilized by high pressure steam at 121℃ for 30min.
[0065] The components in the fermentation broth, fermentation powder and solid culture prepared in this example were determined, and the results ar...
Embodiment 3
[0069] In this embodiment, Acremonium terricola (Acremonium terricola) MKL18 is used as the fermentation object for fermentation to obtain fermentation broth, fermentation powder and solid culture; except that the medium used for liquid fermentation is different from that of Example 1, the remaining contents are the same as those in Example 1. Example 1 is the same.
[0070] The medium for seed culture is: 600g white granulated sugar, 400g yeast powder, 200g soybean powder, 150g peptone, 10gMgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0071] The culture medium for expansion is: 6000g white granulated sugar, 4000g yeast powder, 2000g soybean powder, 1500g peptone, 100g MgSO 4 , 100g KH 2 PO 4 , dissolved in 200L water, natural pH; sterilized by high pressure steam at 121℃ for 30min.
[0072] The components in the fermentation broth, fermentation powder and solid culture prepared in this example were d...
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