Method for improving Hubei mountain ash leaf polysaccharide alcohol precipitation efficiency by adding inorganic salt

A technology of rowan leaves and inorganic salts is applied in the field of polysaccharide extraction, which can solve the problems of low alcohol precipitation polysaccharide yield and the like, and achieve the effects of improving polysaccharide alcohol precipitation yield, convenient operation and reducing alcohol precipitation time.

Inactive Publication Date: 2018-07-27
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low yield of traditional alcohol-precipitated polysaccharides, and to provide a method of addin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for adding inorganic salts to improve the alcohol precipitation efficiency of Sorbus barba leaf polysaccharides, comprising the following steps:

[0023] Step 1: Ultrasonic-assisted extraction of Hubei Sorbus leaves under certain conditions;

[0024] Step 2: Separating the extract from step 1 into solid-liquid to obtain a supernatant containing polysaccharides;

[0025] Step 3: Concentrating the supernatant of the polysaccharide obtained in Step 2;

[0026] Step 4: adding ethanol four times the volume of the concentrated solution and a certain amount of inorganic salt to the concentrated solution in step 3, and carrying out alcohol precipitation;

[0027] Step five: filter the alcohol precipitation solution in step four, and freeze-dry the filter residue to obtain the extracted polysaccharide.

[0028] Said step 1: Ultrasonic-assisted extraction of Hubei Sorbus leaves under certain conditions The specific steps are: take Hubei Sorbus leaves and grind them, pa...

Embodiment 2

[0034] Get Hubei Sorbus leaf and pulverize, cross 60 mesh sieves, get Hubei Sorbus leaf powder; Take by weighing 5 grams of Hubei Sorbus leaf powders that pass 60 mesh sieves, add 150mL distilled water according to material-liquid ratio 1:30, 60 ℃ in Ultrasonic assisted extraction at 350 watts for 40 minutes. After the extraction was completed, the supernatant was obtained by suction filtration. The supernatant was concentrated to 10 mL. After adding ethanol four times the volume of the concentrated solution, the alcohol precipitation system was added with a mass fraction of 1.2% chloride Sodium, alcohol precipitation for 12 hours, suction filter polysaccharide filter cake, freeze-dry polysaccharide, polysaccharide yield 3.47%.

Embodiment 3

[0036] Get Hubei Sorbus leaf and pulverize, cross 60 mesh sieves, obtain Hubei Sorbus leaf powder; Take by weighing 5 grams of Hubei Sorbus leaf powders that pass 60 mesh sieves, add 150mL distilled water according to material-liquid ratio 1:30, 60 ℃ in Ultrasonic-assisted extraction at a power of 350 watts for 40 minutes. After the extraction was completed, the supernatant was obtained by suction filtration, and the supernatant was concentrated to 10 mL. After adding four times the volume of ethanol of the concentrated solution, an alcohol precipitation system with a mass fraction of 1.6% of chloride Sodium, alcohol precipitation for 12 hours, suction filtered polysaccharide filter cake, freeze-dried polysaccharide, polysaccharide yield 4.04%.

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Abstract

The invention relates to a method for improving the Hubei mountain ash leaf polysaccharide alcohol precipitation efficiency by adding inorganic salt. The method is characterized in that by comprisingthe following steps that 1, Hubei mountain ash leaves are subjected to ultrasonic-assisted extraction under certain conditions; 2, extraction liquid in the first step is subjected to solid-liquid separation to obtain polysaccharide supernate; 3, the polysaccharide supernate obtained in the second step is concentrated; 4, ethanol being four times volume of the concentrated liquid and a certain quantity of inorganic salt are added into the concentrated liquid in the third step; then, alcohol precipitation is performed; 5, alcohol precipitation solution in the fourth step is subjected to filtering the filter residue is freeze-dried to obtain the polysaccharide. The method has the advantages that the polysaccharide alcohol precipitation yield can be effectively improved; the waste of the extraction liquid is reduced; the process is simple; the operation is convenient; the cost is low; the method is applicable to large-scale application.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for increasing the alcohol precipitation efficiency of polysaccharides from Sorbus chinensis leaves by adding inorganic salts. Background technique [0002] Polysaccharides are natural compounds enriched in plants, animals, and microorganisms. They have various pharmacological activities, such as antioxidant, immunomodulatory, and antitumor activities, and are widely used in medical treatments, functional foods, and pharmaceuticals. [0003] Sorbus fruit is rich in vitamins and sugar, which can be used for jam, fruit cake and wine making. The seeds contain fat and amygdalin, which are used for soap making and pharmaceutical industry; the bark contains tannins, which can be used in the tanning industry. Utilization, twigs and leaves can be used as fodder. It was found that there is a small amount of polysaccharide in its leaves, its antioxid...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 吉文丽杜梦扬文沛瑶李秀信
Owner NORTHWEST A & F UNIV
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