Method for promoting hepatoma carcinoma cell apoptosis and inhibiting hepatoma carcinoma cell proliferation

Abstract

The invention belongs to the technical field of hepatoma carcinoma cells in the cell therapy field, and particularly relates to a method for promoting hepatoma carcinoma cell apoptosis and inhibitinghepatoma carcinoma cell proliferation. The method comprises the following steps: culturing the hepatoma carcinoma cells until 80% of fusion, and performing the secondary culture, selecting well-grownsub-culture cells, culturing the cells by using cell culture fluid containing astaxanthin calcium alginate microspheres, finally performing the cell proliferation, cell apoptosis and real-time PCR experiment comparison detection of an experimental group and a control group. Under the intervention of astaxanthin, the hepatoma carcinoma cells have an apparent apoptosis phenomenon, and the hepatoma carcinoma cell proliferation is apparently inhibited, and a glycolytic cycle and a tricarboxylic acid cycle in a hepatoma carcinoma cell metabolic process are changed, so a stronger function of inhibiting the hepatoma carcinoma cell proliferation is expressed as a whole.

Description

technical field [0001] The invention belongs to the technical field of liver cancer cells in the technical field of cell therapy, and specifically relates to a method for promoting the apoptosis of liver cancer cells and inhibiting the proliferation of liver cancer cells. Background technique [0002] HepG2 cell line is an ideal tumor model commonly used in the study of liver cancer in vitro, so HepG2 cell line was chosen as a cell model of liver cancer cells in this study. The existence of the carbon-carbon conjugated double bond long chain and the unsaturated α-hydroxy ketone connected in the molecular structure of astaxanthin makes astaxanthin have a strong electronic effect, providing electrons to free radicals and reacting with them, thereby Scavenging free radicals and playing the role of anti-oxidation, due to its own advantages, it has a good application prospect in cancer treatment. However, due to the biological limitations of astaxanthin itself, it hinders the ex...

AI Technical Summary

Problems solved by technology

However, due to the biological limitations of astaxanthin itself, it hinders the exploration of its anticancer effect and anticancer mechanism. In order to effectively explore the therapeutic potential and therapeutic methods of astaxanthin in cancer treatment, it is urgent to explore the Anti-cancer effect and anti-cancer mechanism, making it effective in cancer treatment

[0003] At present, the aerobic glycolysis hypothesis is the most recognized in the metabolic process of cancer cells. Aerobic glycolysis is the main metabolic mode of tumor cells. Glycolysis provides the main carbon precursors required for the synthesis of nucleic acids, proteins, fatty acids, etc. Cancer cells Through the rapid glycolysis process, the energy supply during the rapid proliferation of cancer cells is completed, and the intermediates produced by the glycolysis process are used as carbon precursors for newly proliferating cells, thereby hindering the subsequent step of glycolysis, the tricarboxylic acid cycle (the main energy supply process of normal cells)

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