Method for evaluating prevention and control effect of fungicide on black spot of sweet potato seedlings

Abstract

The invention discloses a set of method for evaluating a prevention and control effect of fungicide on black spot of sweet potato seedlings. According to the method disclosed by the invention, growthconditions of the sweet potato seedlings in a large field are simulated to the greatest extent through an inoculation method comprising the steps of mixing soil or other matrix materials with a sweetpotato black spot conidiospore suspension solution and inoculating, and a cultivation method adopting a cultivation box or an artificial climate chamber, so that uniform disease infection of each treated sweet potato seedling can be ensured and conditions of evaluating the prevention and control effect of the fungicide can be met. Meanwhile, all testing conditions also can be adjustable and controllable; compared with a field experiment, the success rate and accuracy of the experiment are extremely improved and the experiment period is shortened; the disadvantage that the difference between atraditional indoor bioassay result and an actual application result is great is greatly improved. According to the method disclosed by the invention, grading of the disease infection severity degree of the sweet potato seedlings is obtained through a lot of investigation and summarization, and actual conditions of pot culture inoculation are met. Especially, disease infection of sweet potato seedling stem sections and tail wounds are innovatively and independently graded and evaluated, and the method has an important promotion effect of comprehensively evaluating the prevention and control effect of a medicament.

Description

technical field

[0001] The invention relates to an effect evaluation technology of agricultural fungicides, in particular to a set of methods for evaluating the effect of fungicides on the control of potato seedling black spot. Background technique

[0002] Sweet potato black spot occurs in all sweet potato producing areas in the world, and it is common and serious in potato areas in my country. The loss of sweet potato yield caused by the disease is 5%-10% every year, and the loss is 20%-50% when the damage is serious, or even higher (Chinese crop pests and diseases (third edition). Beijing: China Agricultural Press, 2015, 848). In addition, diseased potatoes can produce toxic substances such as ipomeamarone and other furanoids, which can cause poisoning and even death after being used by humans and livestock.

[0003] Causes such as soil bacteria and diseased and healthy mixed species can cause the infection of healthy potato seedlings, and if the potato seedlings are in...

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