Method for evaluating prevention and control effect of fungicide on black spot of sweet potato seedlings

A fungicide and black spot technology, applied in the field of evaluating the effect of fungicides on potato seedling black spot control, can solve the problems of many uncontrollable factors, unstable results, long test period, etc. cycle, the effect of improving the success rate

Active Publication Date: 2018-08-07
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many uncontrollable factors in the field test, the test period...

Method used

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  • Method for evaluating prevention and control effect of fungicide on black spot of sweet potato seedlings
  • Method for evaluating prevention and control effect of fungicide on black spot of sweet potato seedlings
  • Method for evaluating prevention and control effect of fungicide on black spot of sweet potato seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the relation test of different inoculation concentrations and potato seedling susceptibility rate

[0046] Prepare concentration 5×10 5 spores / mL, 1×10 6 spores / mL, 1.5×10 6 spore / mL conidia suspension, inoculate different concentrations of conidia suspension according to the diseased soil preparation method as different treatments, 10 potato seedlings were cut in each nutrient pot, and each pot seedling was a repetition, and each treatment set 4 repetitions. After the cuttings are completed, put the nutrient bowl into the tray, put it in a 25°C incubator and cultivate it for 7 days, and then investigate the infection status. The results are shown in Table 1 and Table 2.

[0047] Table 1 Effects of different inoculation amounts on the susceptibility of potato seedling stems

[0048]

[0049] Note: Different lowercase letters after the data in the same column indicate significant differences at the 0.05 level, and different uppercase letters indicat...

Embodiment 2

[0053] Example 2. Comparison of control effects of potato seedlings treated with different concentrations of chlorothalonil against black spot under potted and hydroponic conditions

[0054] There are 6 treatments in total in the test, including blank control (no fungicide treatment) and 5 different concentrations of chlorothalonil agents, the concentrations of chlorothalonil are: 300mg / L, 400mg / L, 500mg / L, 600mg / L , 700mg / L. Each treatment was replicated 4 times, and each replicated had 10 potato seedlings. The potato seedlings were repeatedly placed in different concentrations of chlorothalonil liquid, and the seedlings were soaked at 25°C for 6 hours.

[0055] According to the method of embodiment 1, pot culture inserts each repeated cutting into a nutrient bowl and cultivates, and the conidia suspension inoculation concentration of diseased soil is 5 * 10 5 spores / mL. The hydroponic method is to transfer each repetition of potato seedlings to a separately placed spore s...

Embodiment 3

[0062] Embodiment 3, different concentrations of thiophanate-methyl treatment potato seedlings to the control effect test of black spot

[0063] There were 6 treatments in total in the test, including blank control (without fungicide treatment) and 5 different concentrations of thiophanate-methyl agents, the concentrations of thiophanate-methyl were: 300mg / L, 400mg / L, 500mg / L , 600mg / L, 700mg / L. Each treatment was replicated 4 times, and each replicated had 10 potato seedlings. The potato seedlings were placed in different concentrations of thiophanate-methyl medicinal solution according to the repetitions, and the seedlings were soaked at 25°C for 6 hours; according to the method of Example 1, each repeated cutting was inserted into a nutrient pot for pot culture, and the meristem of the diseased soil The inoculum concentration of the spore suspension was 5×10 5 spores / mL; after 7 days, investigate the black spot susceptibility and calculate the control effect, the results ...

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Abstract

The invention discloses a set of method for evaluating a prevention and control effect of fungicide on black spot of sweet potato seedlings. According to the method disclosed by the invention, growthconditions of the sweet potato seedlings in a large field are simulated to the greatest extent through an inoculation method comprising the steps of mixing soil or other matrix materials with a sweetpotato black spot conidiospore suspension solution and inoculating, and a cultivation method adopting a cultivation box or an artificial climate chamber, so that uniform disease infection of each treated sweet potato seedling can be ensured and conditions of evaluating the prevention and control effect of the fungicide can be met. Meanwhile, all testing conditions also can be adjustable and controllable; compared with a field experiment, the success rate and accuracy of the experiment are extremely improved and the experiment period is shortened; the disadvantage that the difference between atraditional indoor bioassay result and an actual application result is great is greatly improved. According to the method disclosed by the invention, grading of the disease infection severity degree of the sweet potato seedlings is obtained through a lot of investigation and summarization, and actual conditions of pot culture inoculation are met. Especially, disease infection of sweet potato seedling stem sections and tail wounds are innovatively and independently graded and evaluated, and the method has an important promotion effect of comprehensively evaluating the prevention and control effect of a medicament.

Description

technical field [0001] The invention relates to an effect evaluation technology of agricultural fungicides, in particular to a set of methods for evaluating the effect of fungicides on the control of potato seedling black spot. Background technique [0002] Sweet potato black spot occurs in all sweet potato producing areas in the world, and it is common and serious in potato areas in my country. The loss of sweet potato yield caused by the disease is 5%-10% every year, and the loss is 20%-50% when the damage is serious, or even higher (Chinese crop pests and diseases (third edition). Beijing: China Agricultural Press, 2015, 848). In addition, diseased potatoes can produce toxic substances such as ipomeamarone and other furanoids, which can cause poisoning and even death after being used by humans and livestock. [0003] Causes such as soil bacteria and diseased and healthy mixed species can cause the infection of healthy potato seedlings, and if the potato seedlings are in...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 张德胜田雨婷王永江王爽秦艳红乔奇白瑞英
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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