A cell concentration system and method

Abstract

The invention discloses a cell concentration system and method, comprising: a sample bottle, including a bottle body and a bottle cap matched with the bottle body, a filter device is arranged in the bottle body, and a first cavity is formed by the bottle body, the bottle cap and the filter device Body, the bottle bottom of the bottle body and the filtering device form a second cavity; the loading mechanism can transfer the sample bottle; the concentration and mixing mechanism can pierce the bottom of the bottle, and perform suction and reverse blowing operations on the sample bottle; And the controller, after the controller controls the loading mechanism to transfer the sample bottle to the concentration position, the controller controls the concentration and mixing mechanism to pierce the bottom of the bottle and enter the second cavity, and repeatedly perform the suction operation and reverse air blowing operation. Since the cell concentration system of the present invention is used to carry out cell concentration by performing suction operation and reverse blowing operation, it can effectively prevent the filter device and concentration needle from clogging the sample bottle, and can effectively reduce the concentration of cells while accelerating cell concentration. Cells are superimposed, thereby improving the quality of cell enrichment.

Description

technical field [0001] The invention relates to the technical field of medical bioassays, and more specifically relates to a cell concentration system and method. Background technique [0002] In medical testing, it is often necessary to enrich a specific cell in the specimen, and then identify the cells. In order to ensure the accuracy of the test and the number of cells required for the test, it is usually necessary Enrichment is carried out under certain conditions, which can prevent infection and pollution, and ensure the safety of operators and the surrounding environment. [0003] At present, liquid-based products on the market adopt two methods for cell enrichment: one is to enrich cells by centrifugation after shaking and mixing. The operation process is as follows: manually shake the sample in the vortex shaker, and then manually place the mixed sample on the sample transfer machine to transfer the sample to the centrifuge tube, and then put the centrifuge tube int...

AI Technical Summary

Problems solved by technology

Because the cells are directly adsorbed on the membrane by negative pressure, the cells will be unevenly distributed on the surface of the membrane. When the cells on the membrane are directly pressed on the glass slide, there is a risk of cell overlap in the preparation of the slide.

Although this method saves a lot of manual operations, the overlap and loss of cells is uncontrollable

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