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Highly-accurate immunoturbidimetric fibronectin detection reagent

A fibronectin and detection reagent technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of one-way immunodiffusion method and immune rocket electrophoresis, which are cumbersome in operation, poor in specificity of immune turbidimetry, and inconsistent in detection results. Accuracy and other issues to ensure stability, improve specificity and accuracy, and prevent reagent corruption

Inactive Publication Date: 2018-08-24
济南百齐生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting fibronectin are mainly immunological methods, including one-way immunodiffusion method, immune rocket electrophoresis method, ELISA method, and immunoturbidimetric method, among which the operation of one-way immunodiffusion method and immune rocket electrophoresis method is relatively cumbersome. , it is inconvenient to use in clinical practice. With the promotion of enzyme immunoassay and automatic biochemical analysis instruments, ELISA and immune turbidimetry are widely used in clinical practice, and immune turbidimetry is more expensive than ELISA in terms of price. It has a very big advantage, so it is easier to promote the immune method in ordinary hospitals. However, due to the poor specificity of the immune turbidimetric method itself, it is easy to cause inaccurate test results. Therefore, an immune turbidimetric method with high accuracy is invented. Fibronectin detection reagent is of great significance, so I invented a high-accuracy immunoturbidimetric Fn detection reagent

Method used

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  • Highly-accurate immunoturbidimetric fibronectin detection reagent
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  • Highly-accurate immunoturbidimetric fibronectin detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The reagent composition of a high-accuracy Fn detection reagent described in this embodiment is as follows:

[0037] Reagent R:

[0038] Phosphate buffer 50mmol / L

[0039] Polyethylene glycol-6000 40g / L

[0040] Hydroxylamine sulfate 10mmol / L

[0041] Sodium chloride 9g / L

[0042] PC-300 0.5ml / L

[0043] Protein stabilizer AEP-HBC 3g / L

[0044] Polyoxyethylene lauryl ether 0.3g / L

[0045] Tween-80 1ml / L

[0046] Goat anti-human fibronectin antibody 5ml / L.

[0047] 2) The method of using the reagents in this example:

[0048] The Fn detection reagent described in this example is used in conjunction with a fully automatic biochemical analyzer, such as Hitachi 7180 fully automatic analyzer, etc., and is measured by the one-point endpoint method, and a standard curve is established by using a multi-point calibration method, and the reagent R is placed Go to the corresponding reagent position, place the distilled water, standard and sample in the corresponding posit...

Embodiment 2

[0050] The reagent composition of the FN detection reagent after a key raw material is added described in this embodiment:

[0051] Phosphate buffer 50mmol / L

[0052] Polyethylene glycol-6000 40g / L

[0053] Hydroxylamine Sulfate 20mmol / L

[0054] Sodium chloride 15g / L

[0055] PC-300 1ml / L

[0056] Protein stabilizer AEP-HBC 5g / L

[0057] Polyoxyethylene lauryl ether 05g / L

[0058] Tween-80 5ml / L

[0059] Goat anti-human fibronectin antibody 10ml / L.

[0060] 2) The method of using the reagents in this example:

[0061] The Fn detection reagent described in this example is used in conjunction with a fully automatic biochemical analyzer, such as Hitachi 7180 fully automatic analyzer, etc., and is measured by the one-point endpoint method, and a standard curve is established by using a multi-point calibration method, and the reagent R is placed Go to the corresponding reagent position, place the distilled water, standard and sample in the corresponding position of the sam...

Embodiment 3

[0063] Comparison of the immunoturbidimetric method of the present invention for detecting fibronectin and the ELISA method for detecting fibronectin of the present invention

[0064] Correlation experiment: Using the prepared reagents in Examples 1 and 2 as the experimental group, a commercially recognized reagent for detecting serum fibronectin by ELISA was selected as the control group, and a comparative test was carried out to detect 20 clinical serum samples at the same time , the detection result is as Figure 5 shown. And obtained the correlation curve of the two reagents (such as figure 1 and figure 2 As shown), the test results show that the correlations between the test results of the reagents of Examples 1 and 2 and the control group reagents are 0.9983 and 0.9990, respectively, indicating that there is a great correlation.

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Abstract

The invention relates to a highly-accurate immunoturbidimetric fibronectin detection reagent, and belongs to the technical field of clinical in-vitro detection. The reagent is a single reagent, and the reagent mainly comprises a buffer, polyethylene glycol-6000, a masking agent, an ion balance agent, a preservative, a protein stabilizer, a surfactant, a goat anti-human fibronectin antibody and thelike. The highly-accurate immunoturbidimetric fibronectin detection reagent has high accuracy for detecting the content of fibronectin in blood, and the reagent is simple in composition, can be wellcombined with the automatic biochemical analysis instrument to realize rapid detection, and is very beneficial to clinical application and promotion.

Description

technical field [0001] The invention relates to a highly accurate immunoturbidimetric fibronectin detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Fibronectin (Fn) is a high-molecular glycoprotein with non-immune opsonization and various biological activities, which is composed of two almost identical subunits linked by disulfide bonds. Cerebrovascular disease: The determination of plasma Fn is helpful for the differential diagnosis of cerebral thrombosis and cerebral hemorrhage, and the dynamic observation of plasma Fn content in patients with cerebrovascular disease is helpful for detecting the recovery and curative effect of the disease. Pulmonary diseases: Plasma Fn decreased significantly in the acute phase of cor pulmonale, extremely decreased in the exacerbation phase, and gradually recovered in the remission phase; plasma Fn decreased significantly in patients with severe respiratory failure, pulmon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/82
CPCG01N21/82G01N33/6893G01N2333/75G01N2800/085G01N2800/12G01N2800/222G01N2800/224G01N2800/2871
Inventor 罗维晓胡晓飞王美丽
Owner 济南百齐生物技术有限公司
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