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Primers and probes for synchronously detecting goat origin and cow origin in raw milk as well as kit

A kit and goat technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of less multi-channel and multi-source detection, and achieve the effect of reducing mismatches

Active Publication Date: 2018-08-31
XILINGOL VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technical research, testing standards, invention patents and commercial kits related to the authenticity of dairy products are mainly focused on single-channel single-source detection and heterogeneous quality control detection, while multi-channel multi-source detection and same-tube quality control detection Less reported

Method used

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  • Primers and probes for synchronously detecting goat origin and cow origin in raw milk as well as kit
  • Primers and probes for synchronously detecting goat origin and cow origin in raw milk as well as kit
  • Primers and probes for synchronously detecting goat origin and cow origin in raw milk as well as kit

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] 1. Detection method:

[0064] (1) Extract the DNA of the raw milk and set up an extraction blank control (the control group for the extraction method will be used later).

[0065] (2) Detect the concentration and quality of DNA, and dilute the concentration to 100-200ng / μL.

[0066] (3) Use multiple fluorescent quantitative PCR primers and probes to amplify and detect DNA dilutions, use goat and cow positive standards as positive controls, sterile deionized water as negative controls, and use DNA extraction blank controls as For the control group of the extraction method, the Real-time PCR reaction system is shown in Table 1, and the Real-time PCR amplification parameters are shown in Table 4.

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Abstract

The invention discloses primers and probes for synchronously detecting goat origin and cow origin in raw milk as well as a kit. The sequences of the primers and the probes are as follows: the sequenceof a goat origin detection forward primer is as shown as SEQ ID No.1; the sequence of a cow origin detection forward primer is as shown as SEQ ID No.2; the sequence of a goat origin detection reverseprimer is as shown in SEQ ID No.3; the sequence of a cow origin detection reverse primer is as shown in SEQ ID No.4; the sequence of a goat probe is as shown in SEQ ID No.5; the sequence of a cow probe is as shown in SEQ ID No.6; and the sequence of a quality control probe is as shown in SEQ ID No.7. The primers, the probes and the kit have high specificity and high sensitivity, can realize same-tube detection on goat origin and cow origin in the raw milk and quality control, and can perform quantitative detection on goat origin and cow origin.

Description

Technical field [0001] The invention belongs to the technical field of food detection, and particularly relates to the field of detection of animal origin in raw milk. Background technique [0002] Dairy products are an important part of the human diet, rich in nutrients such as protein, fat, carbohydrates, vitamins and minerals (including trace elements). These nutrients are not only the basis of the macroscopic human body and microscopic cells, but also the source of regulating human life activities and intracellular biochemical reactions. In addition to the rich conventional nutrients in dairy products, the sensory experience of excellent color, aroma and taste is the core of global cuisine. However, with the increasing production and consumption, dairy products have become the main targets of fraud and adulteration in food production, processing, distribution, and catering. [0003] The research on the authentication technology of dairy products in my country started late, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6888C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/107C12Q2545/114
Inventor 郭梁郭元晟巨艳鲁铁冯伟廖成松雅梅钱俊平孙建萍朱建军
Owner XILINGOL VOCATIONAL COLLEGE