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Proteins for breast cancer risk assessment and application thereof

A breast cancer and protein technology, applied in the field of tumor treatment, can solve the problems of patient prognosis, survival time and whether there is recurrence or not

Inactive Publication Date: 2018-09-04
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the same subtype, there are significant differences in the prognosis, survival time, and recurrence of different patients.

Method used

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  • Proteins for breast cancer risk assessment and application thereof
  • Proteins for breast cancer risk assessment and application thereof
  • Proteins for breast cancer risk assessment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Prepare paraffin sections of breast cancer tissue.

[0144] A two-step immunohistochemical kit (GTVision TM III) Staining ATG5 and FIP200 in it. Anti-ATG5 rabbit polyclonal antibody 10181-2-AP (Proteintech Group, Chicago, IL, USA) was used to detect ATG5. FIP200 was detected using rabbit anti-FIP200 polyclonal antibody 16172-1-AP (Proteintech Group, Chicago, IL, USA). For negative controls, the primary antibody was replaced with phosphate buffered solution. The paraffin sections were immersed in xylene for 5 minutes, 3 times. Take out the slices and put them in 100% absolute ethanol for 3 minutes, twice; put them in 90%-70% ethanol at different levels for 3 minutes each. Rinse 3 times with PBS, 3 minutes each time. After 10 minutes of antibody incubation, antigen was extracted by boiling the sections in 0.01M Tris-sodium citrate buffer at 121°C for 20 minutes. For ATG5 and FIP200, in order to eliminate endogenous peroxidase activity, they were incubated with bloc...

Embodiment 2

[0169] According to Example 1, it is found that the second breast cancer prognosis assessment model is better than the first breast cancer prognosis assessment model. Specifically, the second breast cancer prognosis assessment model is called the combined model, and the calculation formula of this prognosis assessment model is:

[0170] m combined =-0.69x S Age +0.62xS Menopausal Status –1.24x S PR status +0.85xS Lymph node status -0.74x SI ATG5 –0.74xS FIP200

[0171] Among them, S Age Refers to the variable value divided according to the patient's age, if the patient's age is greater than 50 years old, then S Age The value is 2, if the age of the patient is less than or equal to 50 years old, then S Age The value is 1; S Menopausal Status is the variable value divided according to whether the patient is menopausal, if the patient is menopausal, then S Menopausal Status The value is 2, if the patient is not menopausal, then S Menopausal Status The value is ...

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Abstract

Belonging to the field of tumor treatment, the invention in particular relates to proteins for breast cancer risk assessment and application thereof. The inventor of the invention detects the expression levels of ATG5 (autophagy-related gene 5) and FIP200 (family kinase-interacting protein 200) in samples to identify samples with low expression of ATG5 and FIP200, and also can further predict thesamples as the high risk and poor prognosis type. In addition, the expression data and survival data of 200 breast cancer patient samples confirm that characterization of the expression of ATG5 and FIP200 can effectively predict high risk and poor prognosis samples, therefore ATG5 and FIP200 have high industrial utilization value in preparation or screening of drugs or kits for diagnosis of breastcancer, or screening of drugs for treatment of breast cancer.

Description

technical field [0001] The invention belongs to the field of tumor treatment, and in particular relates to a protein for risk assessment of breast cancer and its application. Background technique [0002] At present, cancer is one of the main diseases that endanger human health. The number of deaths due to tumors in my country is nearly 2 million every year. Breast cancer is one of the most common malignant tumors in women worldwide, and it is also an important cause of death in women. In my country, like most other countries, breast cancer has become the most common cancer among Chinese women; the number of new breast cancer cases and deaths in China account for 12.2% and 9.6% of the world's population, respectively, and about 210,000 new cases each year , the growth rate of the incidence of breast cancer in my country is 1-2 percentage points higher than that of developed countries. With the application of new technologies and drugs, the cure rate of early breast cancer c...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6893G01N33/57484G01N2800/365G01N2800/7028
Inventor 王鹏裴雨晨薛梦竹刘雨薇
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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