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Application of polydatin in preparation of medicine for preventing and treating Parkinson's disease, and pharmaceutical composition

A technology for resveratrol glycosides and Parkinson's disease, which is applied in the field of biomedicine and can solve the problems that the biological and pharmacological properties of Pol have not been studied in depth.

Pending Publication Date: 2020-05-22
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the biological and pharmacological properties of Pol have not been studied in depth, and there are few reports on the mechanism of Pol that can effectively relieve PD cells, related indicators and symptoms of animal models

Method used

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  • Application of polydatin in preparation of medicine for preventing and treating Parkinson's disease, and pharmaceutical composition
  • Application of polydatin in preparation of medicine for preventing and treating Parkinson's disease, and pharmaceutical composition
  • Application of polydatin in preparation of medicine for preventing and treating Parkinson's disease, and pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Determination of cell viability

[0040] Using PrestoBlue TM Cell viability was analyzed with Cell Viability Reagent (Invitrogen, Carlsbad, CA). In short, the PrestoBlue TM Dilute 10-fold with fresh medium and add directly to cells. After incubation at 37°C for 1 hour, the absorbance was read at 570 nm using a microplate reader (Infinite M200 Pro, Tecan, Switzerland). Cell data are expressed as percentage of untreated control cells. The higher the total metabolic activity, the greater the absorbance value.

Embodiment 2

[0042] Pol can alleviate rotenone-induced cytotoxicity, oxidative stress and mitochondrial damage

[0043] 1. Determination of intracellular ROS levels

[0044] SH-SY5Y cells were seeded in 10mm glass-bottom culture dishes. When the cells grew to about 60%, the cells were pretreated with Pol for 6 hours, and then induced with rotenone for 24 hours. Then the cells were washed three times with PBS, the medium containing 10 μM DCFH-DA probe was added and incubated in an incubator for 30 min, and then the cells were washed three times with PBS, and imaged under a confocal microscope.

[0045] 2. Seed SH-SY5Y cells on a six-well plate, wait for the cell density to be about 70%, and use Lipofectamine TMIn 2000, cells were transfected with pRK5-myc-synphilin-1 and pRK5-myc plasmids in FBS-free medium. After 3 hours of incubation, the medium was replaced with fresh complete medium, and the transfected cells were grown overnight to allow expression of the plasmid. After treatment wi...

Embodiment 3

[0047] Measurement of Oxygen Consumption Rate (OCR)

[0048] OCR was measured on a Seahorse XF24 analyzer (Seahorse Bioscience, USA) following the manufacturer's instructions. Briefly, cells were seeded into 24-well XF Analyzer cell culture plates at 20,000 cells / well. The cells were then washed with XF assay medium (pH adjusted to 7.35-7.45) supplemented with 10 mM glucose, 2 mM glutamine and 1 mM sodium pyruvate, and then incubated at 37 °C in a non-CO 2 Place in the incubator for 1h. Thereafter, 1 µg / mL of oligomycin, 0.5 µM of carbonyl cyanid-4-(trifluoromethoxy)phenylhydrazone (FCCP) and 0.5 µM of Rot / antimycin A were sequentially added. OCR was recorded by the sensor cartridge and analyzed using Seahorse Wave desktop software. The data obtained were normalized to the number of cells per well and expressed as OCR in pmol / min.

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Abstract

The invention discloses an application of polydatin in preparation of a medicine for preventing and treating Parkinson's disease, and a pharmaceutical composition, and relates to the new application of the polydatin, in particular to the application of the polydatin in preparation of the medicine for preventing and treating Parkinson's disease and the pharmaceutical composition. Researches show that the polydatin is applied to preparation of the medicine for preventing and treating Parkinson's disease. The invention shows that oxidative stress, mitochondrial dysfunction and autophagy defects are key mechanisms of a PD model induced by Rot and Parkin knockout. Pol can be used for inhibiting oxidative stress and mitochondrial dysfunction in an SH-SY5Y cell PD model. The knock-down of Atg5 can partially block the protection effect of the Pol on the damage caused by Rot induction. It is possible that an mTOR-Ulk pathway rather than a Parkin pathway participates in the Pol-induced autophagy. The Pol can be used as an effective inhibition factor for dopamine energy and mitochondrial dysfunction in a Parkin-knockout drosophila Parkinson's disease model. Therefore, the application of the polydatin in preparation of the medicine for preventing and treating the Parkinson's disease can play a positive role.

Description

technical field [0001] The invention relates to a new application of resveratrol glycosides, in particular to the application and pharmaceutical composition of resveratrol glycosides in the preparation of drugs for preventing and treating Parkinson's disease, belonging to the field of biomedicine. Background technique [0002] Parkinson's disease (PD) is a relatively common degenerative disease of the central nervous system, characterized by degeneration of dopaminergic neurons in the nigrostriatal compact. PD mostly occurs in middle-aged and elderly people, and tends to be younger. Therefore, the prevention and treatment of PD are in line with the national conditions of my country's aging population. A growing number of studies have shown that oxidative stress, mitochondrial dysfunction, and autophagy defects play important roles in the development of PD. [0003] Recent studies have shown that polyphenols have potent neuroprotective capabilities through multiple potential...

Claims

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Application Information

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IPC IPC(8): A61K31/7034A61P25/16
CPCA61K31/7034A61P25/16
Inventor 李林李鑫柏桦丁亚琦张伟石振雄韩旭唐和舟彭勃黄维
Owner NORTHWESTERN POLYTECHNICAL UNIV
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